الفهرس 
Cover Page Title in EnglishOnly 14 pages are availabe for public view
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Abstract
Salmonella is an important foodborne pathogen, causing high medical and economical costs. Despite the established instructions and measures for preventing salmonellosis in the developed countries, these considerations are lacking in the developing countries. Chicken meat has been identified as one of the most important sources of pathogenic Salmonella serovars. The present study was aimed to determine i) the prevalence of Salmonella in chicken meat and their products, ii) the susceptibility of the isolates to antibiotics, iii) the efficacy of using a six genes-based multiplex PCR to detect and identify the Salmonella serovars Three hundred chicken meat samples were analysed using conventional methods. The isolates were identified, serotyped, and tested against 16 antibiotics. Isolated Salmonella serovars were further analysed for the presence of six target genes (invA, avrA, stn, fliC, stm and sefA) using two multiplex PCR assays. 2.5%, 0.5% and 0.5% of raw chicken meat were positive for S. Typhimurium, S. Kentucky and S. Enteritidis, respectively. All isolates were resistant, at least, to three antibiotics. Multiplex PCR gave 284 bp (invA), 422 bp (avrA) and 617 bp (stn) fragments belonging to the genus Salmonella, and additional three fragments of 559 bp (fliC), 915 bp (stm) and 310 bp (sefA) which allowed distinct identification of S. Typhimurium, S. Kentucky and S. Enteritidis. This study demonstrates the presence of multidrug-resistant serovars of Salmonella from retail chicken meat. The developed six genes-based multiplex PCR in this study is effective of for discrimination of the most common Salmonella species