الفهرس 
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Abstract
Dilated cardiomyopathy (DCM) is the most common cardiomyopathy, accounting for approximately 55% of cardiomyopathies. DCM is characterized by LV dilation and global systolic dysfunction (EF < 50%), in the absence of coronary artery disease or other identifiable causes (such as systemic hypertension, valve disease, drugs, inflammatory heart diseases) capable of causing that magnitude of impairment and is the most common indication for cardiac transplantation. Three major factors have been implicated in the pathogenesis of myocardial damage in DCM: preceding viral myocarditis, autoimmunity, and underlying genetic predisposition Macrophage migration inhibitory factor (MIF) is a pleiotropic cytokine involved in the regulation of innate and adaptive immunity. MIF is known to be a proinflammatory cytokine involved in macrophage and t-cell activation, IgE synthesis, insulin release and carbohydrate metabolism. MIF gene polymorphisms are associated with increased plasma concentrations of MIF, increased risk and severity of inflammatory disease, and reduced response to glucocorticoid medication.
The aim of this work is to study association of MIF -173 G/C gene polymorphism with dilated cardiomyopathy in children.
This study included 50 individuals 28 males and 22 females, their ages ranged between 1-18 years. The studied individuals were divided into two groups, group I included 30 patient having dilated cardiomyopathy (16 males and 14 females), with age ranged between 1-18 years and group II included 20 apparently healthy age and gender
Summary
matched individuals as a control group (12 males, 8 females), their age ranged between 2-18 years.
All patients were submitted to the followings: History taking, clinical examination, echocardiography and laboratory investigation. These include: measurement of serum uric acid, serum Na and determination of MIF G/C genotypes by PCR–RFLP. All controls were subjected to determination of MIF G/C genotypes by PCR–RFLP. The results of this study revealed that:
♦ Regard genotype distribution, The CC genotype was higher in patients compared with controls. While GC and GG genotypes were higher in controls in a comparison with patients and there were statistically significant differences in genotype distribution between both groups (p>0.05).
GC genotype is more risky than GG ”reference group” by1.02.While CC genotype has no odds ratio as CC genotype in control was zero.
♦ Regarding allele frequencies in the studied group, The C allele was more represented in patient group than in control group . While, G allele showed higher frequency in control group compared with patient group . Hence, there were statistically significant differences in the distribution of both allele (C & G ) between patients & controls (p>0.05).
Our study showed that C allele is more risky than G allele by 2.31.
♦ Rearding echocardiographic parameters, there were statistically highly significant difference between different genotypes and EF, FS(p>0.001).
Regarding LVEDD &LVESD, there were statistically highly significant difference between CC & GC genotypes and between CC & GG genotypes(p>0.001). While there were no statistically significant difference between GC & GG genotypes(p<0.05).
Summary
♦ There were statistically highly significant difference between different genotypes in patients as regard serum Na level (p>0.001).
♦ Regard serum uric acid, there were no statistically significant difference between different genotypes in patient group (p<0.05).
♦ There was significant negative correlation between serum sodium level & EF%, LVEDD, LVESD; while, there was no significant correlation between serum sodium level &FS%.