الفهرس 
Material and MethodsOnly 14 pages are availabe for public view
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Abstract
A total of 120 random samples of meat products such as luncheon,
sausage, minced meat and beef burger (30 of each) were collected from
different supermarkets at Gharbia Governorate.The collected samples
were transferred directly to the laboratory in an ice box under complete
aseptic conditions. The samples were immediately examined
bacteriologically for the detection of B.cereus.The results revealed that
the incidence of B. cereus was 20%, 36.37%, 56.67% and 40% in
luncheon, beef burger, Minced meat and sausage, respectively.
All samples were cultured at 37°C for 24 hours aerobically on
selective media (PEMBA) for isolation and purification and on sheep
blood agar to observe the haemolysis properties. The suspected colonies
were examined for their colonial morphology, microscopical
examination, and biochemical reactions.VITEK2 BCL Card used for
more identification of B. cereus isolates.
B. cereus isolates were highly sensitive to vancomycin, rifampicin
and clindamycin. But all isolates were resistant tetracycline, pencillin,
oxacillin, cephalexin, ampicillin and amoxycillin + clavulanic acid.
PCR results revealed that all isolates of B. cereus were positive for
HBL gene which were amplified at 1092 bp. On the hand 3/4 of the tested
strains were positive for cytK gene which amplified at 421bp.