الفهرس 
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Abstract
A survey was undertaken for bacterial isolates capable of producing
bioagents from different Egyptian soils .These isolates were tested against
various species of microorganisms (G+, G-, spore formers , yeast and fungi ) using
inhibition zone determination technique .
One or more of the pathogenic bacterial strains used as tested organisms
were affected by antimicrobial substances produced by bacterial isolates as
indicated by inhibition zones formation . Effect of temperature and heating time,
proteolytic enzyms on cell-free supernatants viability of Bacillus isolates were
determind . The effect of antimicrobial substances produced by bacterial isolates
were compared with the effect of 11 antibiotic disks as reference . The effect of
medium composition, incubation temperature, and incubation period were
investigated the results showed that the best production with nutrient broth at 30-37 0C for 24 – 48 hrs. The most effective isolates against the tested organisms
were identified by API 50 CHB system ansd 16s rRNA, which indicate that these
isolates were Bacillus licheniformis, Bacillus amyloliquefaciens, and Bacillus
subtilis . Mutual antagonistic effect of Bacillus strains was investigated .
Bacillus strains used as a producer for antitumor secondary metabolites in
plant . Determination of minimum inhibition concentration (MIC) of the
antimicrobial substances produced by the chosen five bacterial isolates against a
number of microorganisms were determined .
Whole sediment produced by the five bacterial strains were found to be safe
when using as ration for albino rats.