الفهرس 
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Abstract
The current study revealed that the protective levels of humoral antibody responses were observed at 21th, 28th and 35th days old in chickens of groups II, III, IV, and V but the geometric ELISA mean titre(s) of the groups IV and V had the highest statistically significant (P value ≤0.05) compared with the groups II and III at 21th, 28th and 35th days old. The chickens in group IV had the highest statistically significant (P value ≤0.05) at 35th days old compared with chickens in group V. Both groups II and III had approximately the same geometric ELISA mean titers at 21th, 28th and 35th days old. It is here we showed that the combination between inactivated H5N1 vaccine and oral administration of Purple Coneflower and dandelion in drinking water were able to induce high and fast humoral immune response in broiler chickens compared to vaccination alone. In this experiment, the up regulation of the IRF7 transcript level was detectable at the different time points in the groups II, III, IV and V , existed as early as 15thdays old and remained up to 45th days old compared with control group . The IRF7 transcript level of the group IV had the highest statistically significant ((P value ≤ 0.05) at the different time points compared with other groups. However, in groups II, III and V, the up-regulation in IRF7 transcript level was transient and had no statistically significant with other groups at the different time points. Regarding MX1 antiviral gene, the vaccinated groups were exerted up regulations in the MX1 transcript level at the different time points compared with control group. The highest significant MX1 transcript levels were observed in group IV (P value ≤0.05) at 16th days old and no significant up regulations were observed in other groups at the different time points. These results which may be attributed to the effect of both inactivated AIV vaccine and natural antiviral agents