الفهرس 
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Abstract
The present study was carried out on (1622) rabbits digestive tracts and freshly obtained fecal samples to estimate the prevalence of Eimeria species in rabbits in two successive ages ,(838) samples were from animals 2-3 months old and( 784 ) from 3-4 months old , samples were collected from poultry slaughter shops and markets .The samples were examined during a period extended from the beginning of January 2013 to the end of December 2013. The examined samples were fresh droppings ,intestinal contents and smears from liver cut surface and gall bladders .
The prevalence of Eimeria species was studied in rabbits of ages ( 2-3) months and ( 3-4) months month old,. The results revealed that out of (838) examined samples from ( 2-3) months old rabbits, (429) rabbits were infected and the prevalence of identified Eimeria species was (51.2%) . Nine species of Eimeria were detected in the infected samples; they were E. stiedae, E.exigua, E.perforans , E.intestinalis ,E.media , E.flavescens , E.coecicola ,E.magna and E.irresidua, while the results revealed that out of (784) examined samples from ( 3-4) months old rabbits ,(322) rabbits were infected with eight species Eimeria; they were E. stiedae, E.exigua, E.perforans, E.media , E.flavescens , E.coecicola ,E.magna and E.irresidua and the prevalence of identified Eimeria species was (41.%) .
The prevalence of E.stiedae was(6.44%&7.7%),E.exigua was (10.9%&8.3%),E.perforans(45.3.%&38.6%),E.intestinalis(30.31%&0.0%),E.media(37.4%&32.5%),E.flavescens(6.8%&1.9%),E.coecicola(31.4%&21%), E.magna(28.3%&23.2%) and E.irresidua(7.6%&7.1%) in (2-3) and ( 3-4) months old rabbits respectively. The Seasonal dynamics of Eimeria infection in rabbits were studied , it reached its maximum values in Winter recording (74.8% & 58% ) then it decreased in Spring recording (63%&52.35%) and continue to decrease in Summer recording (45.7%&34%) reaching its lower value in Autumn ( 22.9% &18.2%) in (2-3) and ( 3-4) months old rabbits respectively.
The prevalence of infection of pure hepatic coccidiosis among infected rabbits was (6.3% &5.9%),intestinal coccidiosis was(86.24%&81.37%) while mixed both hepatic and intestinal coccidiosis were(7.5%&12.73%) in ( 2-3) and ( 3-4) months old rabbits respectively.
The rate of infection with one Eimeria species was(6.29%&8.7%), two Eimeria species was(0%&16%), three Eimeria specie was (12.36%&7.76%),four Eimeria species was (51.5%&68.02%), and with five Eimeria species was (30.3%&0%) among infected (2-3) months old rabbits and (3-4) months old rabbits respectively. While the rate of infection with one Eimeria species was(7.06%), two Eimeria species was(6.92%),three Eimeria specie was (9.32%),four Eimeria specie was (59.39%) and with five Eimeria species was (17.31%) among infected rabbits of both ages .
The morphological features of un sporulated and sporulated oocysts of identified Eimeria species were studied, also sporulation of identified Eimeria species was briefly studied, The un sporulated and sporulated oocysts were studied and photographed by color digital camera microscope using the oil immersion lens.
For molecular identification of Eimeria stiedae by using polymerase chain reaction PCR, collection of pure Eimeria stiedae oocysts was done by experimental infection of (10) ”coccidia-free” New Zealand White male rabbits, six weeks-old, with (100000) sporulated Eimeria stiedae for each rabbit, Eimeria oocysts were collected , purified and sporulated in sterile Petri dishes.
Molecular identification of Eimeria stiedae in mixed fecal samples by using(PCR),was conducted using species-specific primers and prepared Eimeria stiedae DNA as a control positive .
Molecular analysis for the partial sequences obtained by (PCR)was done and the sequences produced significant alignments with two Eimeria stiedae isolate in Gen Bank with accession numbers JQ328190. &HM768890.