الفهرس 
Cover Page Title in EnglishOnly 14 pages are availabe for public view
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Abstract
This study performed on 225 cow with clinical mastitis. Sample taken from quarter which appear swollen and harden under aseptic technique, so cleaning with 70% alcohol and first strip of milk was discarded then put sample in falcon tube in ice box. Freshly milk sample were divided into 3 part: first part submit for microbiological analysis another part for isolation of somatic cell the last part for real time PCR and this for determination of the role of interleukin in bovine mammary gland infected with E.coli.In the laboratory were taken all precaution to prevent contamination of the firstly sample part which submit for microbiological analysis, were incubated for 24 hours after addition of in the broth to encourage growth of microbe then streaked on media to know first if it Gram positive or Gram negative after that isolate streaked on media specific for E.coli after growth of bacterial staining colony. It and seen under microscope then confirmed by biochemical tests which specific for E. coli so that confirmed that bacteria in mastitic milk sample was E. coli. another part which used for isolation of somatic cell should not pasteurized and taken not after 24 hours from taken the sample from animals for accurate counting somatic cell at Ekomilk system cell .The last part of sample used for real time PCR which needed more precaution for good results firstly centrifuged the sample and take the cell pellet then wash it three time by buffer. Used the kit for the extraction of RNA after these step taken pure nucleic acid and primers RT PCR Syper green master mix with specific amount then put all chemicals needed for real time reaction in real time system and adjusts thermal profile The system operated for about forty cycles then result curves indicated the occurring of expression of interleukine . And this for determination of the role of interleukin in bovine mammary gland infected with E.coli. This study concluded that: 1-Importance of interleukin-4, interleukin-6, interleukin-8 and interleukin-10 in bovine mammary gland infected with E.coli. 2-More over explained the relationship between interleukin-4, interleukin-6, interleukin-8 and interleukin-10 in bovine mammary gland infected with E.coli. 3-The role of somatic cell in bovine mammary gland infected with E.coli for detection of mastitic animal. 4-Isolation and identification of the causative bacteria E.coli causing mastitis and proving its pathogenicity. 5-Quantification of the IL-4, IL-6, IL8 and IL-10 by R.T PCR. Important results:1-Finally from the obtained results E. coli had an incidence as acausative pathogen for mastitis. 2-The somatic cell consider indicator parameter of udder health and infection. 3-The somatic cell count has significant role to estimate level of infection in case of mastitis spescialy concerning the isolated microbe: E. coli. 4-The somatic cell count when increase this indication for infection in the udder. 5-Cytokine marker have significant role in bovine mammary gland infection and defence. 6-E.coli down regulate interleukin-4 . 7-While E.coli was able to up regulate interleukin-8 which responsible for attraction of other inflammatory cells to the site of inflammation. 8-Interleukin-8 in this study consider highly gene expression than another cytokine of bovine mammary gland infected with E.coli. 9-Different serotype of E.coli cause mastitis. 10-IL-8 consider chemotactic factor. 11-IL-6 consider proinflamatory cytokine.