الفهرس 
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Abstract
A total number of 60 Clarias gariepinus fish obtained from Ismailia governorate and its tributaries were collected from three locations {El-Teraa, El-Berkaa, El-Rashah}.These locations derived from Mohamed Ali channel which derived from River Nile. The fish and water of control group were obtained from central laboratory for Aquaculture research, El-Abbassa, Abo-Hamad, Sharqia, Egypt. Water analysis of the studied polluted locations revealed high level of ammonia. Exposure to ammonia caused decrease in Hb concentration coupled with decrease in haematocrit value and red blood cell counts are obvious indications of anemia. The hematological indices MCHC, MCH, were lowered, while MCV was higher than control group. The total white blood cell counts and the differential white blood cell counts were decreased except for the eosinophils in which there was increased. Serum biochemical examination revealed hypoproteinemia, hypoalbuminemia and hypoglobulinemia with increase in serum ALT, AST, total bilirubin, direct bilirubin, indirect bilirubin, glucose, urea, creatinine and serum ammonia level in the three groups compared with control group.