الفهرس 
Competition of ions for metal- binding sitesOnly 14 pages are availabe for public view
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Abstract
specially the concentration of pollutants such as heavy metals
and phosphorus was varied from fraction to another. One hundred and twenty two microbial (bacterial
and fungal) cultures were isolated from waste fraction, soil, tap water and air. These isolates
and another ten identified yeast cultures did not show any growth on collected waste fractions
except in presence of essential nutrients. Waste fraction of synthetic detergent exerted the more
pronounced deleterious effect on test organisms. The exerted inhibitory effect increasing with
the increasing of waste concentration. Bacterial isolates tolerated highest concentrations of this
waste fractions, while Streptomyces and most fungal isolates were highly sensitive. Only
Pseudomonas flourescons B84, Streptomyces S27, Fusarium A9 and Candida tropicalis CT2 grew on media
containing high levels of these fraction.
Growth curves of microbial isolates in presence of waste
fractions showed the same trend. The tolerant isolates showed highest specific growth rate and gave
higher amount of final net growth on media containing the final detergent waste as compared to
control. Such stimulatory effects were accompared with higher levels of carbon utilization and
yield factor during the first 48-72 hours of incubation. Biomass of tolerant isolates were
autalyzed in the final factory waste and the rate of
autolysis decreased gradually with the elapsing oftime. The efficiency of bioremoval of heavy
metals from final waste varied and was more pronounced by using biomass previously starved for
several times. The most active isolates were Pseudomonas jluorescens B84, Candida tropicalis CT2
and Fusarium A9. The highest accumulation oflead and iron being were accumulated by unstarved
biomass of Ps. jluorescens B84 after 2 and 96 hours,respectively , the total manganese and copper
content of the final detergent waste were completely exhausted by starved biomass of Fusarium
A9 (biomass ii &
biomass iii) while 76.76 % of zinc was removed by biomass iii •
of Candida tropicalis CT2.
The final waste supported growth of mixed cyanobacterial and algal culture. Higher amounts
ofbiomass and greater removal levels of heavy metals were showed in bioreactor as a batch culture
as compared to static batch culture. In case of c:ontinuous culture the optimal condition for
growth and bioremoval of pollutant were 0.012 h-1, 35 °C, pH=8 and agitation ;;peed of 100 rpm.
This condition increased the accumulated heavy metals by 2.38 ,1.58 ,1.24
,1.77and 2.1 I folds for lead, zinc, copper, manganese and iron, respectively as compared to that
accumulated by static culture technique