الفهرس 
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Abstract
Calf diarrhea is a multifactorial disease related to a combination of host and pathogen factors. The most common virus pathogens found in diarrheic calves are rotavirus, coronavirus that causes increased mortality and morbidity among calves. Primary infection of newborn calves with either one of these viruses can cause severe intestinal alterations and diarrhea. Rotaviruses can produce high-morbidity outbreaks of diarrhea in calves under 10 days of age. Morality is variable mainly owing to secondary bacterial infections and electrolyte imbalances. Vaccination is the most important methods for controlling of corona and rotavirus among calves. So, this study aimed to preparation and evaluation of vaccine for rotavirus and coronavirus by using new oil adjuvant for prevention of the dangerous effect in calves and choosing of the most effective vaccine against them. The evaluation of these prepared combined inactivated vaccines was conducted in laboratory. -Sterility and purity testes, where the obtained results proved that the vaccines were pure and free from any viable microorganisms (viral, bacterial, mycoplasma and fungal). - Safety tests was performed on laboratory animals (guinea pigs) and calves and the results revealed that there was no abnormalities or death among the laboratory animals or elevation of temperature and appearance of any clinical abnormalities in calves during the observation period. So, the results indicates the safety of the prepared vaccines. Potency tests were conducted in lab on calves. Different tested animals were divided into fife groups(A,B,C,DE) three calves for each group injected with IA70,ISA50,ISA206,Aluminum hydroxide gel and control group respectively . A total of different adjuvants were evaluated. ISA50, ISA70, ISA206 were obtained from Seppic (France) and formulated according to manufacturer instructions. One serological experiment (SNT) is developed to estimate the immune response against the two viruses. The test (SNT) was conducted on the sera collected from all experimental animals vaccinated and control in certain period. The result revealed that the antibody titer among the different vaccinated groups of the calves against rotavirus and coronavirus. The higher antibody titer for both viruses observed in the group Inoculated with ISA70, followed by ISA50, ISA 206, aluminum hydroxide gel and all of them higher than that of the control group. Also, the result indicated that in rotavirus the higher level of antibody titer observed in the group Inoculated with ISA70 as the level of antibody titer increased steadily till the 8th week of experiment which reach the peak log10 2.31 then decreased at 14th and 16th week of experiment to reach log10 2.11 and still decrease till 22th week of experiment with level log10 1.91 then become non protective at 46th week of experiment with log10 0.3. The 2nd level of antibody titer observed in ISA50 vaccine as the level of antibody titer increased steadily till 8th week with level log10 1.61and become stable till 12th week then maximum level at the period from 16th to 20th week of experiment with level log10 1.91 then then become non protective at 40th week of experiment. The 3rd level of antibody titer observed in the group inoculated with ISA206 vaccine as the level of antibody titer increased steadily till it reached its maximum level at the period from 16th to 20th week of experiment with level log 10 1.91 then become non protective at 36th week of experiment. The 4th level of antibody titer observed in the group inoculated ` with Aluminum hydroxide gel vaccine as the level of antibody titer increased steadily till it reached its maximum level at the period from 10th week of experiment with level log 10 1.81 then it decreased after that and become stable at 16th week till 18th week with level log 10 1.4 become non protective in 26th week. In coronavirus the higher level of antibody titer observed in the group treated with ISA70 as the level of antibody titer increased steadily till become stable from 6th week till 10th week with level log10 2.11 of experiment, then increased till reach the peak at 12th week and become stable till 18th week of experiment and decreased till to 24th week of experiment with level log 10 1.81 then become non protective at 46th week of experiment. The 2nd level of antibody titer observed in ISA50 vaccine as the level of antibody titer increased steadily then become stable from 8th week till 14th week with level log10 1.81 then reached its maximum level at the period from 16th to 20th week of experiment with level log10 2.01 then become non protective at 42th week of experiment. The 3rd level of antibody titer observed in the group treated with ISA206 vaccine as the level of antibody titer increased steadily till it reached its maximum level at the period from 14th to 20th week of experiment with level log10 1.81 become non protective at 36th week of experiment. The 4th level of antibody titer observed in the group treated with Aluminum hydroxide gel vaccine as the level of antibody titer increased steadily till it reached its maximum level at the period from 10th week till 12th week with level log10 1.71 of the experiment then it decreased after that and become stable at 18th week till 20th week then become non protective in 26th week. The results cleared that, the ISA70, ISA50, ISA206 and Aluminum hydroxide gel achieve a higher antibody titer than the control group -Also, the results cleared that, the level of antibody in all vaccines used in coronavirus of higher level than that of rotavirus. The level of antibody in both viruses of a higher level in groups Inoculated with ISA70 followed by groups Inoculated with ISA50 , then the group Inoculated with ISA206 vaccine, followed by the group Inoculated with Aluminum hydroxide gel vaccine and all of them higher than that of control group. The results cleared that, the ISA70, ISA50 and ISA206 gave a higher antibody level and a higher protection than that of Aluminum hydroxide gel and control group.