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العنوان
A study of outer membrane protein omps genes for detection of salmonella organisms in poultry farms /
المؤلف
El Sayed, Mai Mohammed Ahmed.
هيئة الاعداد
باحث / مي محمد أحمد السيد
مشرف / أشرف عواد عبدالتواب
مناقش / أحمد محمد عمار
مناقش / أماني إبراهيم البيلي
الموضوع
Salmonella infection in Poultry. Poultry Processing.
تاريخ النشر
2016.
عدد الصفحات
126 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
Veterinary (miscellaneous)
تاريخ الإجازة
01/1/2016
مكان الإجازة
جامعة بنها - كلية الطب البيطري - البكتريا والمناعة والفطريات
الفهرس
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Abstract

Salmonellosis is one of the important bacterial diseases affecting poultry. It is importance is derived from the loss in productivity of affected birds .
Bacteriological examination of internal organs of 250 chicken were checked for the presence of Salmonella in different flocks. Eleven Salmonella isolates were recovered from the examined bird with an overall incidence 4.4%. The percentage of recovery was 5%, 2.5% and 7.1% from layers, broiler and baby chicks respectively.
A total number of 810 internal organ sample were examined for presence of Salmonella from 250 chicken.In most cases Salmonella isolates were recovered from more than one organ among the examined chicken. The highest percentage of recovery was in liver followed by intestine then spleen 4% ,2.4% and 0.8% . Salmonella organisms were recovered from ovary of layers with an incidence3.33%.
Serological identification of Salmonella strains revealed isolation of S.Rissen, S.Enteritidis and, S.Kentuky from layers. S.Enteritidis, S.Lomita, and S.Gallinarum from broilers and .S.Enteritidis, S.Florida, S.Rissen, S.Gallinarum and S.Sontheim from baby chicks.
This study is intended to provide an update on what is currently known about antimicrobial resistance of 11 Salmonella isolates recovered from chicken farm .Most of the isolates were resistant to antimicrobial drugs making antimicrobial sensitivity result critical for successful treatment .Resistance was observed in Vancomycin (100%),followed by Amoxacillin clavulinic and Ceftazidine 81.8%for each, then Ampicillin and Cefotaxime(72.7%) for each and Nalidixic acid 63.6%.on other hand, the most effective antibiotics were Ciprofloxacin, Sulphmethoxine& Trimethoprin, Gentamycin and Azithromycin.
The clinical samples were examined by PCR with specific primers to detect ompA and ompF.
Twenty samples were examined, eleven were positive Salmonella samples and 9 were negative samples by laboratory and biochemical examination.
The results revealed that all samples were positive with ompA as it gave positive results with negative control sample which indicate that ompA is not specific for Salmonella serovars only but present in other gram negative bacteria . Fourteen samples were positive by ompF PCR. IT was clear that ompF is a promising tool for detection of Genus Salmonella.