الفهرس 
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Abstract
cultivation. Tissue culture technique was used for getting highest values of direct shoot
regeneration and plantlets formation from MS-medium supplemented with 0.5 mg NAA/l + 0.5 mg BM and
incubated under suitable conditions. The comparative study of tissue culture, original plant and
Egyptian drug (from this plant) concerning their moisture, crude fibers, ash, minerals,
carbohydrates, nitrogenous compounds, lipids, phenols, and tannins were determined .
GC-technique was used to fractionate and determine unsaponifiable matters, fatty acids and free
amino acids. Also HPLC techniques were used to detect some active materials in alcoholic extracts
from tissue culture, original plants,
and drug were compared their objective fingerprint resulted from two types of alcoholic extracts
with standard substances for identification and extraction.
The bioassay experiments studied the effect of tissue culture plants, original plants and the drug
which was extracted from this plant taking three dosages (the recommended, higher and lower) and
after the fourth and eighth week, hematological parameters (RBCs, WBCs, Hbg, Ht, MCV, MCHC, PLT and
lymph %) and biochemical blood parameters (total lipids, total cholesterol, HDL-
cholesterol, LDL- cholesterol, triglycerides, GOT, GPT, total protein, albumin, globulin, A/G
ratio, creatinine, urea, and glucose) were measured.
At the end of bioassay experiment histopathological slides were
prepared from different organs (in longitudinal and crossmg sections) liver, kidney, and heart.
Direct regeneration and plantlets formation are the best
methods for mass production of shoot regeneration from different parts of Hypericum perforalum L.
No side effects were observed on rats which were treated by this direct regeneration.