الفهرس 
Introduction and Aim of the workOnly 14 pages are availabe for public view
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Abstract
The incidence of type I diabetes is rising worldwide, particularly in young children. Type I diabetes is considered a multifactorial disease with genetic predisposition and environmental factors participating. Currently, despite years of research, there is no consensus regarding the factors that initiate the autoimmune response. Type I diabetes is preceded by autoimmunity to islet antigens, among them the protein glutamic acid decarboxylase, GAD-65.
Pyridoxial 5’-phosphate (PLP) which is the active form of vitamin B6, is formed from vitamin B6 by the action of pyridoxal kinase. Interaction of GAD65 with PLP is necessary for GAD65-mediated synthesis of the neurotransmitter γ-aminobutyric acid (GABA). PLP is also a required cofactor for dopamine synthesis by L-aromatic decarboxylase (L-AADC). Both GAD65 and L-AADC are expressed in pancreatic islets.
The aim of our study was to assess pyridoxal 5’-phosphate level in children newly diagnosed with T1D.
Our study included two groups; group 1; included 50 children newly diagnosed with T1D, randomly selected and had regular follow up in the pediatric endocrinology outpatients’ Clinic, Minia University Children Hospital during the period from December 2014 to April 2015 and group 2; included another 50 children who were apparently healthy, their ages and sex were matched to the diseased group and classified as a control group. They were sibling of the diseased groups to be under the same familial circumstances of the patients` group.
The studied groups were subjected to thorough history taking, clinical examination and laboratory investigations included: blood glucose level (fasting and postprandial), glycosylated Hemoglobin (HbA1c %), fasting C-peptide level and PLP level.
As regard the results of the current study, we found that
The current study found that the mean age was 7.8± 2.9, most of them were Males 64% versus 36% females, 60% from urban with 40% from rural areas only8% had positive FH of T1D.
Concerning the clinical presentation, 50% presented with classical symptom, 38% with DKA, 8% with nocturnal enuresis, 4% were discovered accidently.
Furthermore, the diabetic group had slightly lower BMI than the control group.
Concerning the laboratory parameters there were statistical significance differences between the diabetic group and the control group as regard fasting and postprandial blood glucose, HbA1c and the C-peptide where (p= 0.001, 0.001, 0.001& 0.01) respectively .
Concerning PLP, the diabetic group had significantly lower levels than the control group where (p= 0.03) and there were insignificant weak positive correlation between PLP and fasting blood glucose where (r= 0.186& p= 0.06).
PLP had significant weak and fair positive correlations with postprandial blood glucose and fasting C-peptide where (r= 0.20& p= 0.04 and r= 0.28& p= 0.02) respectively and had significant positive weak correlation with HbA1c % where (r= 0.21 & p= 0.03).