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العنوان
Isolation and identification of yeasts from fishes with special reference to antifungal sensitivity test /
المؤلف
El-Ghamrey, Mona Mohamed Taha.
هيئة الاعداد
باحث / مني محمد طه الغمري
مشرف / محمد طه محمود
مشرف / ياسمين حسنين ابراهيم طرطور
مشرف / ياسمين حسنين ابراهيم طرطور
الموضوع
Microbial sensitivity tests Fishes.
تاريخ النشر
2016.
عدد الصفحات
171 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
البيطري
تاريخ الإجازة
1/1/2016
مكان الإجازة
جامعة الزقازيق - كلية الطب البيطرى - Microbiology
الفهرس
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Abstract

The present work, aimed to identify yeasts isolates that were
isolated from fishes using by phenotypic method, also evaluated the
ability of two techniques (RapID Yeast plus system and molecular
method) in identification of yeasts and tested the isolates against
antifungal drugs.
Twenty seven diseased Oreochromis species, 22 Clarias
gariepinus and 21 Mugil cephalus were collected from fish pond
in El-Dakahlia Governorate.
Investigation of the affected fishes showed hemorrhage on skin,
darkness of skin, and ulceration may necrosis present, roughness of
scales, destruction of fins, erythema at the base of fins, congested
gills, liver was enlarged friable and kidneys were congested .
Samples were collected from skin, gills, liver, kidneys, spleen
stomach and intestine. The higher incidence of yeasts in three fish
species were in skin 33.95% followed by gills 23.25% then internal
organs and lower in kidneys 8.37%.
Mycological examination revealed the isolation of 215 isolates
that expressed by 74, 77 and 64 in Oreochromis spp. Clarias
gariepinus and Mugil cephalus respectively, were represented in
five yeast genera.
Phenptypic methods and enzymatic RapID yeast plus system are
not accurate in identification of most yeast species. RapID yeast plus.