الفهرس 
Introduction and aim of the workOnly 14 pages are availabe for public view
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Abstract
Vulvovaginal candidiasis affects most females at least once during their lives. It is currently the second most common cause of vaginal infections, after bacterial vaginosis. Most cases are caused by C. albicans. Although most patients have no risk factors, frequent infections may be linked to diabetes, corticosteroids, repeated courses of antibiotics or pregnancy.
This study was performed to study the prevalence of different Candida species among vaginal infections in Minia university hospital for obstetrics and gynecology, to evaluate the antimicrobial susceptibility pattern of the isolated Candida species against different antimicrobial agents and to investigate isolates with resistance and sensitive dose dependant susceptibilities to fluconazole for the expression of ERG11 genes by PCR.
A total of 51 Candida isolates were recovered from 110 female patients suffering from vaginitis. Candida albicans (68.6%) was the most prevalent species followed by Candida glabrata (17.7 %) and Candida krusei (13.7%).
Regarding resistance to the studied antimicrobial agents, it was found that the highest sensitivity of C. albicans to an¬tifungal drugs was seen against fluconazole (33 of 35 isolates) followed by, nystatin (32), clotrimazole (31), amphotercin B (28) and was more resistant to itraconazole (only 6 sensitive). Clotrimazole was the most effective against C. glabrata (4of 9) followed by fluconazole (3). All C. krusei isolates were sensitive to nystatin. Boric acid’s MICs ranged from 2500 μg/ml to 40 000 μg/ml. However, The MICs of terbinafine for the strains were 1- ˃128 μg/ml.
Eleven isolates (2 C. albicans, 6 C. glabrata and 3 C. krusei) were resistant and only one C. krusei strain was sensitive dose dependant to fluconazole , ERG11 gene over expression was detected in only 7 (58.3%) of them.
It is concluded that antifungal susceptibility testing should be performed before prescribing treatment. Fluconazole is widely used drug against vaginal candidiasis but showed high resistance it leads to treatment failure.