الفهرس 
Introduction
Bacteriological quality of ready-to-eat variety meatsOnly 14 pages are availabe for public view
 |  | from | 98 |  |  |
| | | from | 98 | | |
Abstract
The present investigation was designed to determine the bacterial quality of 100 ready-to-eat variety meats which represented 25 meals. The collected samples included 25 each of ready-to-eat liver, lung, intestine (mombar) and tripe samples which were obtained from fast food restaurants in Assiut city.
The mean values of the aerobic plate count of the examined ready-to-eat liver, lung, intestine and tripe samples were 4.16 × 107 ± 2.58 × 107, 4.02 × 107 ± 2.49 × 107, 5.51 × 107 ± 2.83 × 107 and 4.91 × 107 ± 2.49 × 107 cfu/g, respectively. Most of the examined ready-to-eat liver (56%), intestine (48%) and tripe (28%) had aerobic plate count within the range 105-˂ 106 cfu/g, while 32% of the ready-to-eat intestine had counts within the range 106-˂ 107 cfu/g. However, non significant variations in the aerobic plate count were observed between the examined ready-to-eat liver, lung, intestine and tripe samples.
Regarding Enterobacteriaceae, the mean counts of the above mentioned ready-to-eat variety meats were 9.19 × 105 ± 5.95 × 105, 9.34 × 105 ± 7.62 × 105, 1.99 × 106 ± 1.64 × 106 and 8.02 × 105 ± 6.29 × 105 cfu/g, respectively. Most of the examined ready-to-eat liver (52%), lung (48%), intestine (44%) and tripe samples (40%) had non detectable counts (˂ 10/g) while 16, 16, 20 and 16% of such samples had counts within the range 103- ˂ 104, 102- ˂ 103, 103- ˂ 104 and 104- ˂ 105 cfu/g, respectively. A non significant difference in the mean Enterobacteriaceae count was noticed between the examined ready-to-eat variety meats.
As for S.aureus, 80% of the examined ready-to-eat liver samples and 88% each of the examined ready-to-eat lung, intestine and tripe samples were contaminated with such organism where the mean count values were 1.28 × 107 ± 9.76 × 106, 5.51 × 106 ± 2.70 × 106, 1.83 × 107 ± 1.23 × 107 and 9.59 × 106 ± 8.60 × 106 cfu/g, respectively. Most of the examined ready-to-eat liver (32%), lung (28%) and intestine (40%) had S.aureus count within the range 105-˂ 106/g, while 40% of the examined ready-to-eat tripe samples had counts within the range 104- ˂ 105/g. variations in S.aureus count between the examined ready-to-eat variety meats were non significant.
Bacteriological analysis of the examined ready-to-eat variety meats meals revealed that their mean aerobic plate, Enterobacteriaceae and S.aureus counts were 1.86 × 108 ± 9.39 × 107, 2.87 × 106 ± 1.71 × 106 and 3.97 × 107 ± 2.11 × 107 cfu/g, respectively. Majority of the examined meals had aerobic plate count (64%) within the range 105- ˂ 106/g, Enterobacteriaceae count (20%) and S.aureus count (48%) within the range 104- ˂ 105/g.
Members of Enterobacteriaceae recovered from the examined ready-to-eat variety meats meals were E.coliO128:H2 (2%), E.coliO26:H11 (1%), Enterobacteraerogenes (6%), Enterobactercloacae (5%), Klebsiellaozaenae (6%), Klebsiellapneumoniae (7%), Proteusmirabilis (5%), Proteusvulgaris (3%), Providencia (3%), SalmonellaEnteritidis (1%), SalmonellaTyphimurium (1%), Serratiamarcescens (10%) and Shigellasonnei (5%).
S.aureus was existed in 80, 92, 96 and 88% of the examined ready-to-eat liver, lung, intestine and tripe samples from which coagulase positive strains constituted 70, 62.2, 54.2 and 59%, respectively. On the other hand, 35.7, 46.7, 30.7 and 61.5% of the recovered coagulase positive S.aureus from the aforesaid variety meats proved to be methicillin resistance S.aureus whereas vancomycin resistance S.aureus failed to be detected in any of the examined samples.
However, the microbiological quality of the examined ready-to-eat variety meats varied according to its bacterial load and presence of some foodborne pathogens. The public health significance of the isolated microorganisms was discussed and the suggestive measures to protect the consumer and produce high quality variety meats meals were outlined.