الفهرس 
Tissue culture and somaclonal variationOnly 14 pages are availabe for public view
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Abstract
Fouad Mamdouh Amin Ashry: Optimization of Regeneration
Protocol and Molecular Assessment of Somaclonal Variations in
Peanut (Arachis hypogaea L.). Unpublished M.Sc. Thesis, Department
of Genetics, Faculty of Agriculture, Ain Shams University, 2016.
This investigation was carried over three successive generations.
Seeds of eight peanut cultivars, i.e., Giza-5, Giza-6, Ismailia-1, Sohag-
104, Sohag-110, R-92, Virginia and Gregory were irradiated with five
gamma ray doses i.e. 0, 50, 100, 200 and 300 Gy and sown at Ismailia
Research station. At the same time, regeneration protocol for peanut was
established. The plantlets were transferred to the greenhouse for
acclimatization process and obtain R1 and R2 regenerates. Evaluation of
M2 and M3 generations were done at Ismailia Research station in the next
two seasons under natural infection with studied pests, i.e., Two-spotted
spider mite (Tetranychus urticae), Ahphids (Aphis craccivora Koch) and
Cotton leaf worm (Spodoptera littoralis) under field conditions. M2 and
M3 generations and R1 and R2 regenerates were evaluated under
artificially infestation conditions with the studied pests. All cultivars
ranged from resistant to moderately susceptible against the studied
economic pests. Meanwhile, three genotypes appeared; one of them,
developed via irradiation with gamma ray doses (CMPM-4), and two
were developed through combination between somaclonal variation and
irradiation with gamma ray doses; CMPM-2 and CMPM-8, they were
resistant to the studied pests. Ten RAPD primers and seven ISSRs primers
across the eight peanut cultivars and the three newly developed resistant
mutants were used to genetically characterize the eleven genotypes and
find out molecular markers associated with pests resistance in peanut.
Several molecular markers either positive or negative related to pests
resistance in some peanut mutants were obtained.
Key words: Peanut, Tissue culture, Gamma ray, pest resistance, twospotted spider mite, aphid, cotton leaf worm, RAPD, ISSRs.