الفهرس 
Introduction and Review of LiteratureOnly 14 pages are availabe for public view
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Abstract
Periodontal regeneration is the ultimate goal of the
periodontal therapy. The current treatment modalities differ greatly
in their outcome. The autogenous bone graft is considered the ”gold
standard” of grafting material. Cell therapy provide a great hope to
regenerate the periodontium with its different structure. Adipose
derived stem cells are attractive source due to their abundance, ease
of access, painless obtaining and biologic effect without ethical or
safety concerns.
The present study was conducted on eight healthy dogs.
Lipoaspirate was obtained by liposuction, ADSCs were isolated and
propagated ex vivo and after 2 weeks the surgical creation of acute
interpromximal one wall periodontal defects were created in a split
mouth design including 4 groups, group A as a control , group B as
autogenous bone graft , group C as ADSCs and group D as
ADSCs/autogneous bone graft group with chitosan scaffolding for
all groups. Sacrifice was done after 3 months and the blocks were
prepared for histologic with (H.&E.) stain and histochemical with
(Masson trichromestain) studies. The histomorphometric study was
conducted on the histochemical slides to obtain the statistical
analysis.
The results of this study revealed formation of new
periodontal structures in all groups with variant degree of
Summary
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maturation which was the least in control group and the greatest in
ADSCs/autogenous bone graft group and these results were
confirmed histochemically. The statistical analysis revealed a
significant difference between ADSCs and ADSCs/autogenous bone
graft groups in coronal area percent measurement and significant
difference between all groups and the control group in apical area
percent measurement. There was no significant difference between
ADSCs group and autogenous bone graft group, while the