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العنوان
Some Micromorphological Studies on the Mucosa of the Oesophagus and Stomach
of the Quail /
المؤلف
Helal, Yasmine Asaad Mansour.
هيئة الاعداد
باحث / ياسمين اسعد منصور هلال
مشرف / السيد مسلم محمد مسلم
مشرف / عبد العليم عبد العليم السبع
مشرف / شيماء حسين محمد حسين
الموضوع
Gizzard. Histology. Histochemistry. Immunohistochemistry.
تاريخ النشر
2016.
عدد الصفحات
112 p. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
البيطري
تاريخ الإجازة
1/1/2016
مكان الإجازة
جامعة القاهرة - كلية الطب البيطري - Cytology and Histology
الفهرس
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Abstract

This study was conducted on fifty apparent healthy adult Japanese quail of both sexes
with average age ranged from (8_ 9) weaks and average weight ranged from (250 _ 300gm).
After slaughtering of quail, small pieces from cervical and thoracic part of oesophagus, crop,
proventriculus and longitudinal section of oesophago _ proventricular junction, proventriculargizzard junction, cranial and caudal sacs of gizzard, middle gizzard and pyloric region were
collected. These organs were examined for histological, histochemical, ultrastructural and
immunohistochemistry. The oesophageal mucosa was lined by stratified squamous non
keratinized epithelium under which the lamina propria contained oesophageal glands. The
proventricular mucosa was thrown into plicae and sulci which lined by simple columnar
epithelium. This epithelium showed positive reaction to Periodic acid- Schiff (PAS), Alcian
blue, Aldehyde fuchsin and Best′s carmine techniques. Compound tubuloalveolar glandular
lobules were located in the submucosa. The gizzard had thick middle part, thin cranial and
caudal sacs. Simple branched tubular glands opened in gizzard pits. The gizzard glands lined by
two type of cells; chief cell and basal cell. While ultrastructural analysis of the cranial sac
revealed three types of cells; mucous cells, chief cells and enteroendocrine cells. On the other
hand the caudal sac revealed two type of cells; mucous cells and enteroendocrine cells. The
Pyloric region characterized by villous appearance of the mucosa. Ultrastructurally, it showed
three types of cells; chief cells, mucous cells and enteroendocrine cells. The enteroendocrine
cells were identified immunohistochemistry by using antibody against Somatostatin, Gastrin,
Glucagon and Serotonin in the stomach. The immunoreactivity of MUC5AC was present in the
lining epithelium of mucosal folds of both proventriculus and gizzard and also in the gizzard
glands, whereas MUC6 reactivity was found in the oxyntico_peptic cells of proventricular
glands.