الفهرس 
Human PapillomavirusesOnly 14 pages are availabe for public view
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Abstract
This is a case control study. This work was carried
out on samples collected from Ain Shams University
from women attending outpatient clinics of Maternity
Hospital (ASUMH) for Recurrent Early Pregnancy loss
(REPL). All included women were admitted to outpatient
clinic between 8-14 weeks gestation for termination of
pregnancy due to absence of fetal cardiac activity or
absence of fetal pole on ultrasonographic examination with
history of two or more consecutive unexplained first
trimestric miscarriage with no live birth.
- Samples:
(A) Study group (Case study), was compiled 60 samples
were divided into Thirty placenta and Thirty urine samples
from the same woman attending outpatient clinics of
ASUMH for REPL.
(B) Control study, was compiled 60 samples were divided
into Thirty placenta and Thirty urine samples from women
presented with first missed miscarriage with no history
of recurrent early pregnancy loss and had at least one previous uneventful pregnancy with no previous
obstetric history of adverse pregnancy outcomes.
All the patients included in this study were subjected to
the following:
1. DNA extraction from Placental and urine
samples.
2. Detection of HPV by conventional PCR.
3. Detection of HPV by nested PCR.
4. Detection of HPV by SYBER green Real
time PCR.
5. Genotyping of HPV by multiplex PCR.
-The results indicated that:
HPV infection was found as 16.6% and 26.7% in
placental samples, 6.6% and 36.7% in urine samples by
using PCR and nPCR or real-time PCR respectively. HPV-
16 was the predominance HPV type in both sample (10%)
followed by HPV-31(6.6%) in both samples and HPVtypes 51 and 18 (3.3%) in both samples. There was 3.3% co
infection (infection by more than one type of HPV) in
placenta and urine samples by types 16 and 51. There was
poor agreement of placenta and urine samples results in generic and a moderate agreement for type-specific
detection of HPV. The results also showed a significantly
difference between number of miscarriage in HPV positive
(3.38±0.744) and HPV negative (2.95±0.385) placenta
samples.