الفهرس 
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Abstract
A total of 775 samples of which 75 were BTM and 700 of farm environmental samples were collected from 5 dairy farms in Kafr-El-sheikh Governorate and Alexandria road, Egypt. Three Governmental farms (A, B and E) and 2 private farms (C and D). The collected samples were examined for the hygienic quality of BTM, the prevalence of some food-borne pathogens and for the antimicrobial resistance of isolated pathogens. The results revealed that the mean values of APC in BTM samples of farms A, B and E were 6.6×105±2.4×104, 2.7×105±3.1×104 & 7.4×104 ±4.7×103 cfu/ml, respectively, while in private farms C and D were 4.7×104 ±3.7×103 and 1.2×104 ±1.7×103 cfu/ml, respectively. The mean values of coliforms in BTM samples of farms A, B and E were 1.6×104 ±1.7×103, 1.4×104 ±2.9×103 and 3×103 ±1.4×102 MPN/ml, respectively, while in farms C and D were 1.3×103 ±3.1×102 and 3.8×102 ±1.4×102 MPN/ml, respectively. The mean values of Staphylococci counts in farms A, B and E were 6.4×104 ±1.6×103, 4.2×104 ±2.2×103 and 3.5×103 ±4.1×102 cfu/ml, respectively, while the count in farms C and D were 2.1×103 ±2.5×102 and 1.7×103 ±3.8×102 cfu/ml, respectively. The mean counts of SCCs/ml in BTM samples of farms A, B and E were 603×103 ±310×102, 396×103 ±163×102 and 169×103 ±140×102 cells/ml, respectively, while in farms C and D were 174×103 ±146×102 and 142×103 ±96×102 cells/ml, respectively. Testing of BTM for quality will provide producers with valuable information concerning the status of health and sanitation in their herds and help to produce high quality BTM.
The examination of BTM and dairy farms environment for prevalence of pathogens using PCR technique indicated that farms A and B were positive for all four concerned pathogens (S. aureus, Salmonella spp., E. coli and L. monocytogenes), farm E was positive for 3 pathogens (S. aureus, E. coli and L. monocytogenes), while only S. aureus and E. coli were isolated from farms C and D. Testing of isolated pathogens against 17 antimicrobial agents revealed resistance and sensitivity of these pathogens in various percentages. The hygienic as well as the public health significance of obtained were discussed.