الفهرس 
Historical background
ComplicationsOnly 14 pages are availabe for public view
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Abstract
Giardia lamblia (Giardia intestinalis or Giardia duodenalis) is a flagellated enteric protozoan parasite, unicellular eukaryotic microorganism that commonly causes diarrheal disease worldwide, and cyst is the stage which is able to exist outside the host in a suitable environment and is the form of the parasite by which Giardiasis is usually transmitted, in Egypt prevalence can reach 44% in some areas.
This study was conducted to evaluate the effect of radioactive Cobalt-60 and 254 nm-UV irradiation on infectivity of Giardia lamblia cysts to mice, and examining their ability to reduce the Giardia lamblia infectivity.
The study was conducted on 60 BALB/c mice, aged four to eight weeks old and. They were divided into six groups. 10 mice in each group.
Parameters used for evaluation of the infectivity of Giardia lamblia cysts include: Stool analysis for the mice on day0, 9, 10, 11. 12 and 21, Duodenal aspiration from mice and examination of the aspirate for the presence of Giardia lamblia cysts or trophozoites. And Histopathological examination of the small intestine and assessment of grade of inflammation and villus architecture.
Among the results we found that the histopathological examination is the most sensitive parameter used in this experiment, with percentage in group (1) includes mice infected with a suspension of human derived Giardia lamblia cysts irradiated with Cobalt-60 , 50% and group (2) includes 10 mice infected with a suspension of human-derived Giardia lamblia cysts irradiated with 254-nm UV rays 20%, followed by duodenal aspirate examination in group (1) 40% and in group (2) 0% and lastly the stool examination which gave with group (1) 20% and no difference with group (2).
The present study highlighted the effectiveness of low dose radioactive cobalt-60 and ultraviolet radiations 254nm in prevention of Giardia lamblia infection and indicated that they can be used as a control measure to prevent infectivity and as a mean of water treatment.