الفهرس 
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Abstract
The increased potential of inulinase applications (production of high fructose) promoted us searching new sources of microbial inulinases. In this study 380 fungal isolate were isolated from 45 soil samples, identified and screened for their inulinolytic potentialities. Among the isolates Aspergillus sclerotiorum Hoba 2143 was the most active fungal strain able to produce considerable amount of inulinase (534.78 Ugds-1), this strain was completely identified on the basis of ITS region sequence analysis together with its phenotypic characteristics and used through out the rest of the study. Conventional and statistical experimental (Response surface methodology) techniques used to optimize fermentation conditions of solid state fermentation using agro-industrial residues (rice straw and Jerusalem artichoke tuber powder) increased inulinase production up to 5721.5 Ugds-1 . The entrapment technique using sodium alginate 2.5% with cross linker glutraldehyde 2% for inulinase immobilization increased the thermodynamic properties of inulinase (optimum temperature: 45°C, Ea: 5 Kcal mole-1 , Ed : 139.32 Kcal mole-1 and T1/2 at 60°C: 15.4 min) in comparison to that of free inulinase (optimum temperature: 40°C, Ea: 6.37 Kcal mole-1 , Ed : 110.22 Kcal mole-1 and T1/2 at 60°C: 2.2 min). The hydrolysis products of inulin by Aspergillus sclerotiorum Hoba 2143 inulinase using TLC technique proved that the enzyme is of Exo-acting type.