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العنوان
MOLECULAR XENOMONITORING OF LYMPHATIC FILARIASIS ELIMINATION IN A SENTINEL VILLAGE IN MENOUFYIA GOVERNORATE, EGYPT/
المؤلف
Hamdy, Dina Mamdouh.
هيئة الاعداد
باحث / دينا ممدوح حمدي
مشرف / مجد حمند براايمه
مشرف / عزة حمند كامل
مشرف / ميال عبد العزيز مصطفى
مشرف / راىما أيم سمير
تاريخ النشر
2016.
عدد الصفحات
214 P. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
الطب (متفرقات)
تاريخ الإجازة
1/1/2016
مكان الإجازة
جامعة عين شمس - كلية الطب - Medical Parasitology
الفهرس
Only 14 pages are availabe for public view

from 214

from 214

Abstract

Lymphatic filariasis (LF) is focally endemic in Egypt where the female mosquito, Culex pipiens, is responsible for its transmission. The aim of the study was to investigate the impact of implementation of 13thround of MDA in two Egyptian villages in Menoufyia governorate area after failing transmission assessment survey (TAS) in 2005.It also aimed at demonstrating the value of supplementation of molecular xenomonitoring (MX) technique to the immunochromatographic card test (ICT)when deciding whether to stop mass drug administration (MDA) in a locality. A cross-sectional study was carried out in the villages in 2014 with two sections. Section (1):a school based survey where all the primary school entrants (6-7) years of age were tested by ICT. Section (2): Mosquito based survey where a total of 152 mosquito pools collected from Samalay and 227 from Kafr El-Tarainah were tested for the presence of the gDNA of Wuchereria bancroftimicrofilaria by Real-time PCR assays. The results revealedthat all primary school children in both villages were 100% negative for antigenemia. Also, all mosquito pools were 100% negative for the microfilarial gDNA.
Key words: LF, Wuchereria bancrofti, Culex pipiens, ICT, MDA, MX.