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Abstract Liver and kidney from all experimental animals were immediately isolated, cleaned from blood adhering matters, washed in ice-cold saline, and dried. Parts from the liver and kidney of each group were sliced and immediately fixed in 10% formalin for histological examination. One fourth gram from each liver tissue was homogenized separately in 2 ml cold buffer (50mM potassium phosphate pH 7.5, 1mM EDTA) per gram tissue using tissue homogenizer (Tekmar model TR-10, West Germany). The homogenate was centrifuged at 4000 rpm for 15 minutes using cooling centrifuge (Hettich model EBA 12R, Germany). Then, the supernatants were stored at -80°C for reduced glutathione (GSH) determination. The remaining of liver and kidney tissues were frozen at -20°C for the other biochemical investigations. |