الفهرس 
Review of LiteratureOnly 14 pages are availabe for public view
 |  | from | 117 |  |  |
| | | from | 117 | | |
Abstract
Pseudomonas aeruginosa is naturally resistant to wide spectrum of antibiotics in current use and
infections by this bacterium have been found very refractory to chemotherapy so in this study we
attempt to study the incidence and resistance pattern of Pseudomonas aeruginosa in farm animals and
fish.
The present study was carried on 157 samples randomly collected from different farms at Alexandria
goveronate as follows:
• 37 sample collected from calve diarrhaeic faecal samples.
• 42 sample collected from infected wound and abscesses from sheep and goats.
• 28 nasal swab samples from cows with abnormal respiratory manifestation and nasal
discharge.
• 50 fish where samples collected from fish liver, kidney, heart, skin and oral cavity of fish.
The samples were directly transferred to laboratory where they were prepared for microbiological
examination for isolation of Pseudmonas aeruginosa, Detennination of antibacterial sensitivity test
and isolation of plasmids.
1- incidence of Pseudmonas aeruginosa among farm animals:
Result of this study showed that I 07 samples were collected in case of farm animals from
calves diarrhaeic faecal samples were 37 samples
from faeces revealed 2 (5.4%) + ve, 5 (17.8%) +ve out of28 nasal discharge sample and 4 (9.5%) +ve
out of 42 infected wound.
These results having a total of II (10.28%) out of 107 examined samples.
2- Incidence of Pseudmonas aeruginosa among fish:
Results of this study showed that 50 samples were collected from fish liver, heart, kidney,
internal gills and skin revealed 4 (8%) +ve isolates of Pseudmonas aeruginosa.
3- The antibacterial sensitivity of Pseudomonas aeruginosa:
3.1. The antibacterial sensitivity ofPseudomonas aeruginosa isolated from farm animals
Results of this study showed that 11 isolates of Pseudomonas aeruginosa· were tested against 8
selected antibacterial agents. The drug sensitivity test revealed that Pseudomonas aeruginosa was
sensitive to gentamicin and amikacin with sensitivity of 100%. On the other hand, the isolates were
resistant to erythromycin, sulphamethoxazole trimethoprim and amoxacillin with a degree of 100%,
100% and 90.9% respectively.
3.2. The antibacterial sensitivity ofPseudomonas aeruginosa isolated from fish:
Results of this study showed that 4 isolates of Pseudomonas aeruginosa were tested against the same
8 selected antibacterial agents which showed that gentamicin was the best antibacterial agent
against isolates of Pseudomonas aeruginosa with sensitivity of I 00%. On the other
hand, the isolates were resistant to flumoquin, erythromycin, amoxacillin and
sulphamethoxazole trimethoprim with an incidence of 100% for all.
3.3. The antibiogram of Pseudomonas aeruginosa isolates tested against some selected
antibacterial agents, revealed that all the I 5 isolates (100%) were sensitive to gentamicin and
had sensitivity ranging from (0-93.3%) to the other antibacterial agents. Also had high resistance
to erythromycin (100%), sulphamethoxazole trimethoprim (100%) and amoxacillin (93.3%) then
flumoquin (80%) than other antibacterial agents used. Although had intermediate sensitivity ranging
from (0-20%) to the selected antibacterial agents.
4- Results of plasmid DNA profile analysis:
9 Pseudomonas aeruginosa isolates were positive for analysis by usmg polyacrylamide gel
electrophoresis. They carried plasmids with molecular weight 20 megadaltons.