الفهرس 
introductionOnly 14 pages are availabe for public view
 |  | from | 97 |  |  |
| | | from | 97 | | |
Abstract
Results of the identification of root-knot nematodes isolate revealed that it was Meloidogyne javanica using the prenial pattern technique as well as using the Molecular Biology SCAR- PCR technique. Sensitivity test of four tomato cultivars infected by M. javanica that cause root-knot. Results showed that the all cultivars infected by the nematode but they differed in the degree of response to injury. The four cultivars could be divided into classes such as sensitive (GS) or weak resistance Elissa, Ferroz and hybrid 737. Ninety-three bacterial isolates from surroundings of root plants (family Solanaceae) were isolated and tested in the lab against second stage larvae. Laboratory tests showed that there were different effects of these isolates on second stage larvae. After bioassay examination, the most effective two bacterial isolates were chosen for further examination. Two isolates were tested on tomato plants to study their effect on plant infection and growth, Results showed that the PGPR isolates increased plant fresh weight and length compared with non-treated plants. Identification the of PGPR isolates by enzymes, as well as through 16s rDNA were performed. Results showed both isolates were identified as: (Bacillus subtilis strain 30L1-2 and B. subtilis strain OCFB3). Results revealed that using B. subtilis strain 30L1-2 and B. subtilis strain OCFB3) have good effect in reducing number of nematode galls, eggmasses and J2 on both tomato cultivar
Key words: Tomato- root-knot nematode- Induction resistance- Beneficial microorganisms