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العنوان
Risks Associated with Listeria Monocytogenes in Raw Milk in Marsa Matrouh Governorate /
المؤلف
Khedr, Abdel Fattah Mohammed Mahmoud.
هيئة الاعداد
باحث / عبدالفتاح محمد محمود خضر
مشرف / عزة محمود كامل حسانين صبيح
مشرف / وليد ماجد المنير
مناقش / عزة محمود كامل حسانين صبيح
الموضوع
Food contamination.
تاريخ النشر
2016.
عدد الصفحات
108 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
Veterinary (miscellaneous)
تاريخ الإجازة
5/11/2016
مكان الإجازة
جامعة كفر الشيخ - كلية الطب البيطري - قسم مراقبة الأغذية
الفهرس
Only 14 pages are availabe for public view

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from 108

Abstract

Virulence of L. monocytogenes is controlled by many factors, yet
inlA, inlB and hlyA genes remain the major determinants of pathogenicity.
These virulence genes are responsible for intestinal adhesion, invasion and
systemic spread of the pathogen within host. So, the four detected isolates
of L. monocytogenes were subjected to molecular identification by PCR
DNA extraction. The four isolates were harbored inlA gene (100 %), while
one isolates was negative for inlB and another isolates was negative for
hlyA.
The results of antibiotic sensitivity test showed that all L.
monocytogenes isolates (100 %) were resistant to Ampicillin, Cephalothin and
Sulfamethoxazole-Trimethoprim and 75% of them showed resistance for
Erythromycin and Tetracycline. However, none of them (0%) resisted
Gentamicin, Kanamycin and Chloramphenicol.
The control of L. monocytogenes in food needs commitments at
different levels of milk production. Milking hygiene habits should be guided
and preparation areas should be cleaned and sanitized before and after use.