الفهرس 
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Abstract
The aim of the present study was designed to evaluate the influence of
hypoglycemic agent (insulin) and immunosuppressant agent
(azathioprine) on pulp tissue healing after pulp capping using MTA,
BioAggregates and calcium hydroxide.
Eight healthy dogs were used for the study and classified into four
groups: [Immunosuppressed (G1); Normoglycemic (G2);
Hyperglycemic (G3); and Control (G4)].
A total of 120 teeth were used (15 teeth in each dog). Four dogs
sacrificed after one month of operative procedure and the other ones
were after two months. The groups were treated preoperatively as
follows: G1 treated by azathioprine for two months. In G2 and G3,
diabetes was induced two weeks preoperatively by I.V. injection of
alloxan; then insulin was given to control blood glucose level (BGL).
The amount of the injected insulin was adjusted to be as close as normal
in G2 (Normoglycemic group) and to high level in G3 (hyperglycemic
group). G4 (control group) had no treatment before and\or after
operative procedure.
Class V buccal cavities were prepared in the teeth approximately 1 mm
coronal to the gingival margin with no. 2 inverted cone carbide at high
speed under copious normal saline irrigation. Sterile sharp probe was
used mechanically to expose the pulp. After controlling the bleeding,
the tested materials were applied as indicated by the manufacturers.
After the end of each experimental period, dogs were euthanized using
20 ml of 5% thiopental sodium solution rapidly injected through the cephalic vein. Bone blocks containing teeth were fixed in 10% formalin
and then decalcified in 5% formic acid. Serial sections of 5 µm
thickness were cut in the bucco-lingual plane. All sections were stained
with hematoxylin and eosin for histopathological evaluation using light
microscope.