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Abstract Total of 640 fecel samples collected from diarrheic and apparent healthy cattle were examined for the presence of Genus Salmonella. Bacteriological examination of fecal samples from diarrheic and apparent healthy cattle revealed the isolation of 12 isolates from 640 with in incidence 1.87 %, 8 isolates from diarrheic cattle with incidence 2.05 % and 4 isolates from apparent healthy cattle with incidence 1.6 %. Serological identification revealed that the most prevalent serovars were Salmonella (Typhimurium, Lomita and Belgdam) to isolates for each and followed by Salmonella (Larochelle, Virchow, Infantis, Derpy, Anatum and Berzany) one isolates for each. It was noticed that 3 fecal samples were positive by RT- PCR more than culture methods, on other hand 2 samples were positive by culture methods and couldn’t be detected by fim A gene RT- PCR Plasmid encoding fimbriae (pef) and virulence associated gene (avr A), virulence genes were detected in 7 and 4 out of 12 culture positive samples. |