Search In this Thesis
   Search In this Thesis  
العنوان
Characterization of Bioactive Compounds and Functional Properties of some Plant Extracts /
المؤلف
Abo El-Maati, Mohamed Fayez Mohamed.
هيئة الاعداد
باحث / محمد فايز محمد ابو المعاطي
مشرف / صلاح الدين محمد
مشرف / علي محمد علي ابراهيم الجابي
مشرف / محمد فوزى رمضان حسانين
الموضوع
Plant extracts. Medicinal plants.
تاريخ النشر
2016.
عدد الصفحات
237 p. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
الزراعية والعلوم البيولوجية (المتنوعة)
تاريخ الإجازة
1/1/2016
مكان الإجازة
جامعة الزقازيق - كـليـــة الزراعـــة - الكيمياء الحيوية الزراعية
الفهرس
Only 14 pages are availabe for public view

from 266

from 266

Abstract

Aromatic and medicinal plants are rich sources of bioactive compounds with health-promoting activities. In this work twenty plants with different organs such as seeds, leaves, flowers, buds … etc. were studied in terms of phenolic compounds content, antioxidant, antibacterial and anticancer properties. The extraction was done using ethanol 80% with assist of ultrasonic and by distilled water with assist of microwave. Total phenolic compounds and total flavonoids were measured using Folin-Ciocalteu reagent and AlCl3, respectively. The highest amount of total phenolic compounds was found in clove extracts followed by thyme. Also, the highest amount of flavonoids was found in the sweet marjoram ultrasonic-assisted extract. Total antioxidant capacity of extracts was estimated by different methods including DPPH˙ (1,1-diphenyl-2 picrylhydrazyl radical), ABTS•+ (2, 2` azino bis-(3-ethylbenzthiazoline-6-sulfonic acid), β-carotene/linoleic bleaching test and ferric reducing antioxidant power (FRAP). Clove extracts exhibited the strongest antioxidant capacity in all assays, followed by thyme, rosemary and cinnamon extracts. In general, ethanol 80% with assist of ultrasonic and distilled water with assist of microwave extracts showed distinctive activity in compare with the synthetic antioxidants TBHQ. Antibacterial activity of tested extracts was assayed using disc assay for Gram positive and Gram negative bacteria (Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 8739, Listeria monocytogenes ScottA, Salmonella enteritidis PT4 and Serratia marcescens). Tested extracts had minimum inhibition concentration (MIC) against tested bacteria. The effect of tested extracts on pathogen tested bacteria was examined using scanning electron microscopy (SEM) and transmission electron microscopy (TEM. Anticancer activity for selected extracts was determined by using cytotoxicity evaluation using viability assay on three cell lines: MCF-7 cells (human breast cancer cell line), HepG-2 cells (human Hepatocellular carcinoma) and M-NFS-60 (Mouse Myelogenous Leukemia). The different extracts of garden cress, mint, clove, rosemary, anise and cinnamon showed a good IC50 on these cell lines.