الفهرس 
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Abstract
In Egypt ,production of fish has significantly increased during the last years, due to the
improvement in culture techniques specially in Oreochromis niloticus farming .
The intensive nature of modern poultry production and processing tends to
concentrate high quality byproducts, and this has stimulated their reuse in fish farms as
good fertilizer with low cost supplementary feeds, chicken manure is preferred because of
its ready ,solubility and high level of phosphorus concentrations.
The microbiological analyses revealed the presence of various pathogenic microorganisms
in manure such as Vibrio bacteria .
for this relations between fish farms and poultry manure we began this research under a
title (Phenotypic and genotypic diversity of Vibrio spp.isolated from Oreochromis
niloticus and /or poultry manure)
In this study we try to put our hands about the fact, if the poultry manure which used as
fish farm feeding consider as a source of fish Vibrio infection or not!
We began our search by collecting 200 samples of fish (Oreochromis niloticus) showing
clinical signs as erosion of fins and hemorrhagic patches over the skin and 20 samples
from poultry manure used as fish farm feeding from Behera and Kafr –Elsheikh
governorates.
There are (62, isolates) from Oreochromis niloticus are positive to Vibrio infection (V.
parahaemolyticus 34 isolates), (V. alginolyticus13,isolates),( V. anguillarum10,isolates),(
V. harveyi 3,isolates)and V. vulnificus only 2 isolates).
While all poultry manure (sablah) samples showing positive to Vibrio infection with only 3
serotypes (V. parahaemolyticus 17,isolates), (V. anguillarum 2,isolates) and finally
V.vulnificus one isolate.
A number of isolates from fish (Oreochromis niloticus) (13,isolates) 5,isolates from V.parahaemolyticus,3 isolates from V.alginolyticus,2,isolates from V.anguillarum,2 isolates from V.harveyi and one isolates from V.vulnificus) are subjected to antibiotic sensitivity test against 9 discs of antibiotics and the result showing that ciprofloxacin is the most susceptible antibiotics to different isolated Vibrio bacterial serotypes , followed by flumequine,,and finally gentamycin, also showing little response to enrofloxacine,Oxytetracyclin .
On the other hand all tested bacterial isolates appeared as resistant to the other tested antibiotics (sulphamethoxazole /trimethoprime , amoxycillin and doxycyclin).
Within a comparison of our results we found that the most common isolates between poultry manure (sablah) and fish samples is V. parahaemolyticus.
So we apply the molecular identification ERIC-PCR on V.Parahaemolyticus isolates from
fish samples (4,samples )and poultry manure (5,samples) to investigate the unique of
DNA bands in both fish and poultry manure used as fish feed by ERIC-PCR test which
done in Faculty of Veterinary Medicine Kafr-Elsheikh University.
The result of ERIC –PCR estimated under observation of our teachers which showing that
there is only one sample DNA alleles in fish have the unique structure with poultry
manure (sablah) within percentage not exceed 11% only.
Although poultry manure showing 100% Vibrio isolates , it play a minor role only11%
from Vibrio infection in fish farms fed on it ,89 % of other Vibrio infections come from
other sources. This study confirm our impression that poultry manure is a good ,safe and
cheaper source as Oreochromis niloticus fish farm fertilizer and can increase the
effectiveness of poultry manure by using antibiotic that Vibrio showing high response to
it.
but we need more new searches in this category due to the broad use of poultry manure in
fish farm feeding .