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العنوان
Effect of NaCl and some amino acids on the production of some secondary metabolites in in vitro cultures of Mentha longifolia =
المؤلف
Salem,Basem Ibrahim Abass.
هيئة الاعداد
باحث / باسم إبراه مٌ عباس سالم
مشرف / علا عبد العز زٌ الشناوي
مشرف / محمد جمال التركي
مشرف / هدى الس دٌ المقدم
الموضوع
Ornamental Horticulture.
تاريخ النشر
2016.
عدد الصفحات
95 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
الزراعية والعلوم البيولوجية (المتنوعة)
تاريخ الإجازة
1/1/2016
مكان الإجازة
جامعة الاسكندريه - كلية الزراعة - الزهور ونباتات الزينة وتنسيق الحدائق
الفهرس
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Abstract

The present study was conducted at the Tissue Culture and Plant Micropropagation Laboratory of Floriculture, Ornamental Horticulture and Landscape Gardening Department, Faculty of Agriculture, Alexandria University, during 2014 and 2015, to study the effect of phenylalanine and sodium chloride on the production of some secondary metabolites in in viro cultures of Mentha longifolia using two explants (shoot tips and leaves).
Leaf segments about 1 cm2 and shoot tips consisting of 3-4 cm long stem segments containing three auxiliary buds (as explants) were planted on MS medium supplemented with NAA (0.25 mg/l) + BAP(2.5 mg/l ) + 30 gm/liter sucrose + 7 gm/liter agar for shoot induction from shoot tips, while MS medium contained 2,4-D (4 mg/l) + BAP (0.5 mg/l ) for callus induction from leaf segments, the pH was adjusted to 5.8±0.02 using 1N NaOH and 1N HCl.