الفهرس 
العنوانOnly 14 pages are availabe for public view
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Abstract
Skin is the first line of defense in the body and can be easily injured with
either external object or with internal blunt force trauma. There are many
types of wound dressings with different properties and mechanism of action
for accelerating healing. They may activate the wound repair, help in the skin
regeneration process, provide the moisture environment for wound or help in
its drying. Biomaterials, the non-drug biologically-derived materials have
become very important means to treat, enhance or replace any tissue, organ
or function in an organism based on their structural rather than biological
properties. For viable translational outcomes, we considered that a hydrogel
made of the 2 polymeric biomaterials; alginate and chitosan alone, with no
additional growth factors, cytokines or cells would prove sufficiency to treat
wound injuries and can act as a scaffold for activating cells migration and
proliferation as well as promoting the angiogenesis. The present study aimed
at preparing a new type of Alginate/ Chitosan PolyElectrolye Complex
(PEC) hydrogel and testing the required wound healing properties of the
hydrogel in vitro which were then tested in vivo with excisional acute wound
models in rats and compared with those of a commercial cream dressing and
non-treated wounded rats. The healing promoting effects were assessed using
different methods including the quantification of expression of two
angiogenesis-controlling genes (VEGF and vWF) and measurement of the
wound closure rate % with histological examinations for skin and wounds
beds. In addition, the effect of gel degradation in the body was monitored by
routine measuring of kidney functions.
The dressed wounds showed maintained suitable levels of the angiogenic
genes for activating hemostasis and accelerating the angiogenic cascades for
maintaining the blood supply to the newly formed skin tissue in the wound
area. Accelerated rebuilding for the layers of wound area was observed
proving efficiency of the hydrogel in the treatment of acute wounds and its
role in the regeneration of the damaged skin tissues. The wound closure rate
was faster with wounds treated with the chosen hydrogel than those treated
with the cream and the non-treated wounds.