الفهرس 
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Abstract
This study aimed to investigate the prevalence of antibiotics residues in farm bulk milk and their public health hazards. A total of 100 farm tank milk samples of different sources were collected from dairy farms in state of Kuwait from a period from January to July 2016. Each raw milk sample was collected in 100 ml sterile capped plastic jar then transferred in an ice box (4°C) to the laboratory of milk hygiene department at Mansoura University with a minimum time of delay for preparation of samples and detection of antibiotic residues. Collected samples screened for presence of beta lactams and tetracycline antibiotic residues by using Delvotest® SP NT and Betastar® combo HS kits. Comparison between both kits was done. Positive screened milk samples analyzed by using High Performance Liquid chromatography (HPLC) for detection of Amoxicillin, Penicillin G, Cephalexin, Tetracycline and oxytetracycline antibiotic residues.The prevalence of antibiotic residues in raw milk samples was 17% using Delvotest® SP NT and Betastar® combo HS kits. The incidence of Beta lactams and tetracyclines in raw milk samples was 6%, 11%, respectively by using both screening test kits and HPLC. Analysis by HPLC revealed that out of 17 positive milk samples 2,1,3,6 and 5 samples from analyzed positive milk samples, the milk samples were contaminated with Amoxicillin, Penicillin G, Cephalexin, Tetracycline and oxytetracycline , respectively. 13out17 positive analyzed samples exceed maximum residue limit which established either by European Union (EU) and Food and Drug Administration (FDA) regulations. Proper control measures and good monitoring system should be activated to ensure that only the safe milk used in dairy production and for human consumption.