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Abstract The present study was divided into four main parts: The first part of the present study concerned with isolation and identification of fungi inhabiting healthy and early blight diseased leaves of tomato plants. Tomato plants were collected from four governorates (Aswan, Qena, Sohag and Assiut), Egypt. Different isolates of the pathogenic fungus Alternaria solani, the causal agent of tomato early blight disease were also isolated from different cultivated tomato fields. All the isolated fungi were morphologically identified at first and then some selected fungal species of the isolated fungi were further molecularly identified based on PCR amplification and sequencing of the ITS region (ITS1, 5.8 S and ITS4). The pathogenicity test for the Alternaria solani isolates was conducted under greenhouse conditions on tomato Geronimo cultivar. The second part focused on extraction, identification and purification of different natural compounds from selected isolated fungal endophytes. The antifungal activities of the isolated natural compounds were tested at different concentrations against the most virulent isolate of Alternaria solani F11 (KT721909) under laboratory conditions.The present study was divided into four main parts: The first part of the present study concerned with isolation and identification of fungi inhabiting healthy and early blight diseased leaves of tomato plants. Tomato plants were collected from four governorates (Aswan, Qena, Sohag and Assiut), Egypt. Different isolates of the pathogenic fungus Alternaria solani, the causal agent of tomato early blight disease were also isolated from different cultivated tomato fields. All the isolated fungi were morphologically identified at first and then some selected fungal species of the isolated fungi were further molecularly identified based on PCR amplification and sequencing of the ITS region (ITS1, 5.8 S and ITS4). The pathogenicity test for the Alternaria solani isolates was conducted under greenhouse conditions on tomato Geronimo cultivar. The second part focused on extraction, identification and purification of different natural compounds from selected isolated fungal endophytes. The antifungal activities of the isolated natural compounds were tested at different concentrations against the most virulent isolate of Alternaria solani F11 (KT721909) under laboratory conditions. |