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Abstract In a trial to investigate the occurrence of ORT in broiler chickens, 250 birds were collected from 30 commercial and backyard farms. The birds were subjected to clinical and postmortem examination. Sera samples were examined by both ELISA and SAT tests. The clinical findings of the examined birds were respiratory troubles ”nasal discharge (100%), rales (83%), swollen eyes (23%) accompanied by diarrhea 46% . Accompanied gross lesions were caseated materials in eyes (33%), Fibrinous pericarditis, perihepatitis and air sacculitis (30%) as well as caseated materials in trachea and bronchi (63%) , foamy yoghurt like exudates was recorded in 10%. On the other hand septicaemia (43%), enteritis and bronzy liver were seen The ELISA results were positive among 80% of the examined flocks. The comparison of SAT test with ELISA test on 49 selected samples revealed 93.9% for ELISA and 69.38% and 89.8% against ORT-1 and ORT-2 antigens respectively. It could be concluded that ORT is one of the main components of respiratory disease complex among broiler chickens. In case of unavailability of ELISA, SAT test using appropriate and commonly circulating serological antigen could be a useful cheap test for field for monitoring the disease specially in early stage of infection. |