الفهرس 
AcknowledgementOnly 14 pages are availabe for public view
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Abstract
Drugs acting on the central nervous system (CNS) are extremely valuable therapeutically because they produce specific physiological and psychological effects. These drugs are used to correct pathophysiological events in the abnormal CNS. The thesis is devoted to the development of new methods for the analysis of certain drugs used for treatment of CNS disorders, namely: topiramate (TOP), levetiracetam (LEV), lamotrigine (LAM), carbamazepine (CAR), oxcarbazepine (OXC), zonisamide (ZON), sulpiride (SUL) and ezogabine (EZO) in pharmaceutical preparations and/or in biological fluids. A general introduction about the CNS acting drugs was included in the first part. A validated spectrofluorimetric method is proposed for the analysis of TOP and LEV, separately in pharmaceutical tablets and in human plasma. TOP and LEV were also simultaneously determined in human plasma using fourth derivative synchronous spectrofluorimetric technique. On the other hand, a simple and reliable HPLC method with UV detection for the simultaneous determination of CAR, LEV, LAM and OXC in human plasma samples was proposed. Also, a validated, rapid, sensitive and accurate HPLC-fluorescence detection method after pre-column derivatization with NBD-Cl was presented for the simultaneous determination of the two AEDs (ZON and TOP) with the antidepressant drug; SUL in volunteers plasma at their therapeutic level with no interference from plasma matrix and any metabolites. Finally, two sensitive, simple and selective spectrophotometric and spectrofluorimetric methods have been developed for determination of ezogabine, levetiracetam and topiramate in their pure and in tablet dosage forms. The proposed methods are based on the condensation of the primary amino group of the three drugs with acetylacetone and formaldehyde according to Hantzsch reaction yielding highly fluorescent yellow colored dihydropyridine derivatives.