الفهرس 
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Abstract
This study was carried out at the Laboratory of Physiology and Biotechnology, Animal Production Department, Faculty of Agriculture, Mansoura University, in cooperation with the International Livestock Management Training Center (ILMTC), Kafr El-Sheikh Governorate, belonging to the Animal Production Research Institute, Dokki, Giza, Egypt, during the period from November 2015 to May 2016. The current study aimed to: 1- Determine of hormonal profile (FSH and LH) in does rabbit serum from three stages of reproductive status 1- from rabbit does premating, 2- at mating and 3- postmating by 12h), 2- The effect of age of rabbit does as a source of ooctytes (5-6 months) and (~1 year) on ovarian structure, categories and maturation rate of rabbit oocytes in vitro, 3. Effect of aging and type of serum (doe rabbit serum, DRS from three stages of reproductive status under two concentration 10 and 20% for each type of serum compared as hormonal supplemented to tissue culture medium (TCM-199) on in vitro maturation rate of rabbit oocytes, and 4. Evaluation of the rate of success in in vitro fertilization with the best results of in vitro mature rabbit oocytes with types of serum, with two rabbit ages. A total of 45 New Zealand white (NZW) rabbit does (12 young does rabbit with around 5.1 months of age and 2.45 kg live body weight (LBW), 12 old does rabbit with approximately one year of age and 3.22 LBW were used in maturation, 9 does for reproductive status serum for (addition to maturation medium and determined FSH and LH levels) and 6 young and 6 old does for the development as well as 3 bucks with average of 8.4 months and 3.78 kg LBW were used in this study. Rabbit does were subjected as donors of oocytes and rabbit bucks were used for semen collection used in in vitro fertilization. Ovaries were collected immediately after slaughtering and oocytes were harvested. All visible follicles on the ovarian surface with ≥1 mm in diameter were counted. The oocyte yield was recorded and the number of oocyte/ovary was calculated. The recovery rate was determined as percentage of oocytes in proportional to total number of visual follicles present on the ovarian surface of each doe and oocyte categories were evaluated. Only morphologically high quality immature oocytes were utilized in this study. Based on the foregoing results concerning donor age, types of serum and level of serum, the current study may conclude that, the best in vitro maturation rate of rabbits oocytes could be obtained, when oocytes recovered from older donors and matured in medium supplemented with 20% post-mating does serum flowed by oocytes matured in medium supplemented with hormone (FSH-LH). While, the best value in cleavage and development rate based on total cleavage oocytes being, in oocytes from old donor and matured in medium with hormone.