الفهرس 
Hepatocellular carcinomaOnly 14 pages are availabe for public view
 |  | from | 149 |  |  |
| | | from | 149 | | |
Abstract
Hepatocellular carcinoma (HCC) is the most common primary liver malignancy and the sixth most common cancer worldwide. Egypt has the highest prevalence of HCV in the world
predominantly genotype 4,which has been attributed to previous public health eradication schemes for schistosomiasis. The resistance to chemotherapy is a major obstacle in the
treatment of hepatocellular carcinoma (HCC),necessitating the discovery of additional agents.Therefore,the aim of present study was focused on the confirmation of the suitability and validity of the new markers would be achieved by demonstrating their significant change and reproducible expression during disease and disease management. HepG2 cell line was used to provide a source for HCC cells. The cell
cultures were divided into four groups: control untreated group, 5–fluorouracil treated group as a standard chemotherapy for hepatocellular carcinoma (positive control), Kochia indica extract treated group and the group treated with a combination of 5-FU and Kochia indica in different ratios.Treatment with Kockia indica extract, 5-fluorouracil and the combined treatment showed a significant cytotoxicity to HepG2 cells, with different IC50 values, when compared to the control.Regarding toxic effect, 5-FU showed IC50 = 237.56 μg/ml which is lower cytotoxic in compared to Kochia indica with IC50 =120.5 μg/ml which emphasize the resistance of HepG2 cell to the 5-FU as a chemotherapy. Present results showed also an additive effect for the combination of both agents; 5-FU and Kochia indica in treatment of HepG2 cells. Concerning to dehydrogenases activity,five of the
dehydrogenases out of six showed a significant decrease in the treated
groups than in the control group. They are ADH,ADHI,ADHII,ALDH,DHDL and AFP which is an important biomarker for HCC
diagnosis and prognosis uptill now