الفهرس 
I. INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
Fresh plant parts of Vicia faba pods and Chicory seeds were bought on March 2013 from a local market at Alexandria, Egypt. The damaged parts were removed and the healthy ones of uniform size and appearance were used in the preparation of Chicory seed oil extract and Vicia faba pods extract.
Classification of the plants: It was mentioned in introduction (page 21).
3.2.1. Preparation of plant extracts
3.2.1.1. Preparation of Vicia faba pods extracts (VE)
Vicia faba pods were purchased from a local seed market in Alexandria, Egypt. VE were prepared using methanol as described by the method of Murthy et al (2002). Vicia faba pods were washed well in running water and were dried in the sun for 96 h at 27○C. The dried pods were ground using coffee grinder. Dried powder was soaked in methanol (1:14
Materials and methods
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w/v) at 25○C for 72 h, and then filtered through double rings filter paper for removal of peel particles. The residue was re-extracted two times with methanol and filtered. The filtrates were pooled and the methanol was evaporated using rotatory evaporator at 40○C to get rid of methanol. The resulting VE was stored in a dark bottle at 4○C until used.
3.2.1.2. Preparation of Chicory seeds extracts (CE)
Chicory seeds were purchased from a local seed market in Alexandria and prepared using ethanol according to the method of Harborne, (1988). Three kilograms crushed seeds were soaked in 95% ethanol and allowed to stand at 25○C for 72 h. CE was concentrated to dryness under reduced pressure in a rotary evaporator at 40○C to get rid of ethanol. The resulting CE was dissolved in dimethyl sulfoxide (DMSO) for administration into the rats and was kept at 4○C until used.
3.2.2. characterization of different plants
3.2.2.1. Determination of phenolic contents in different extracts
Total phenolic contents were determined colormetrically (Taga et al., 1984) since Phenols reduce Folin reagent forming a blue color solution which measure at 750 nm.
Reagents
- Folin-Ciocalteau reagent was dissolved in distilled water (1:1, v: v).
- 2% Sodium carbonate (Na2Co3) prepared in distilled water.
- Standard gallic acid (1 mg was dissolved in 1 ml distilled water).
- VE (1 gm dissolved in 10 ml distilled water) and CE (1 gm dissolve in 10 ml DMSO).
Procedure
One hunderd microliters of Folin-Ciocalteau reagent and 2 ml Na2CO3 were added to 100 μl of each extract separately (sample), or 100 μl of gallic acid (standerd), or 100 μl of distilled water or DMSO (blank) and incubated 2h at 27○C, the blue color solution was measured at 750 nm. Standard gallic acid was used (Table 2). The results were plotted as shown in (Figure 12).
Table (2): Different concentrations of gallic acid and their corresponding absorbance
Gallic acid (mg/ml)
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
Absorbance (750 nm)
0.233
0.527
0.752
0.978
1.335
1.571
1.777
1.953
1.995
Materials and methods
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y = 2.4412x
R2 = 0.9847.