الفهرس 
ConjunctivitisOnly 14 pages are availabe for public view
 |  | from | 183 |  |  |
| | | from | 183 | | |
Abstract
Background: biofilms are composed of bacterial cells living in multicellular structures embedded within a self-produced matrix of extracellular polymeric substance. One of the most important virulence factors Staphylococcus species.
The aims of this study: to determine association of ica A with biofilm formation in staphylococci isolated from patients with conjunctivitis also to determine association of ica A gene with antibiotic resistance profile in staphylococci causing conjunctivitis.
Methods: A total of 50 clinical staphylococcal isolates were included from a total of 100 patients suffering from staphylococcal eye infection with conjunctivitis attended the Ophthalmology outpatient department of the Research Institute of Ophthalmology, Egypt. Biofilm formation ability was determined by microtiter plates and Congo Red Agar methods. PCR was used to detect ica A gene.
Results: Thirty cases (60%) of the isolates were S. aureus and twenty were CoNS (40%). The results showed that weak, moderate and strong cases in both were significant with phenotypic methods MTP and CRA and the presence of ica A gene was prevalent in all cases with biofilm and associated with high MDR. Positive ica A S. aureus were (89%) MDR and positive ica A gene CoNS were (72.3%).
Conclusions: S. aureus and CoNS showed a high biofilm forming ability in conjunctival infections. Both phenotypic methods of biofilm detection showed comparable. Significant association was found between biofilm formation and ica A gene also between MDR staphylococci and ica A gene carriage.