الفهرس 
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Abstract
Neonicotinoids represent the newest major class of insecticides; these insecticides are derivatives of nicotine. Acetamiprid is one of these derivatives. In the present work, the genotoxicity of acetamiprid was treated alone and combined with laser irradiations simulating solar radiations on human lymphocyte cultures .Also, we assess the mutagenic effect of three types of laser irradiations including ultra-violet, visible and infrared which simulate the same action or phenomena induced by sunlight in the open field using chromosomal aberrations (CAs) assay. Results revealed that acetamiprid (Mospilan 20% SP) did not induce any significant induction of CAs in human lymphocytes at all doses (0.01, 0.1, 1, 10, 100 and 1000 ug/ml culture), in comparison to control group. Also results revealed that the individual exposure to UV-excimer laser 308 nm and vis. diode laser 650 nm showed a slight significant increase ( p<0.05) in CAs at high doses. In contrast, infrared diode laser808nm induced a highly significant increase in CAs (p<0.001) at all doses. The results of combined treatments between acetarmiprid and laser irradiations were different. Combined treatment of acetarmiprid and UV-excirner laser 308 nm irradiation indicated a slight significant increase (p< 0.05) in (CAs only at high dose. Combined treatment between acetamiprid and visible-diode laser 650 nm irradiation showed moderately significant increase in GAs only at high dose (p<0.0l). Combined treatment of acetamiprid and infrared diode laser 808nm7 irradiation induced highly significant increase (p<0.001) in the (CAs at all doses. In addition, combined exjiosure of UV-excimer laser 308 nm and vis. diode laser 650 nm showed a slight significant increase in CAs at low dose(<0.05) and relative significant increaseincrease at high dose (p<0.01). The combined exposure of excimer laser and infrared diode laser 808 nm showed a highly significant increase (p<0.001). Also, the combined exposure of vis. diode laser and infrared diode laser showed highly significant increase (p<0.001). Thus, infrared radiation can cause health problems and may he more dangerous than other radiations. It can be concluded that the combined treatments of acetamiprid with laser irradiations, which simulate the main solar radiation in the open field, appeared greater genotoxicity than that caused by acetamiprid alone, It is recommended for agriculture, industry and scientific research to avoid the exposure of acetamiprid with VIS or IR radiation during fabrication, storage, manipulations and applications without following preventive medicine measures. Also, the present work strongly imposes the need of more serious consideration of the potential risk of the exposure to laser.