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Abstract Poultry is one of the most reservoirs of salmonellae that can be transmitted to human through the food-chain causing a high risk of bacterial food poisoning Pathogenisis of salmonella depends on a large number of factors controlled by a cluster of genes responsible for the actual virulence of salmonellae. Furthermore, multidrug resistance of salmonella to commonly used antibiotics is increasing both in veterinary and public health field area and has emerged as global problem that lead to treatment failure Therefore, the outcome of this study to know the current status of motile salmonellae, describe antibiotic resistance profile and asses the value of PCR in rapid detection of some virulence genes among different salmonella serotypes isolated from broiler chicks at different localities in Sharkia Governorate. In the present work, bacteriological examination of 150 flocks with a previous history of whitish diarrhea and exceeding mortality rate revealed 49 salmonella positive flock (32, 66%). The highest rate of salmonella species isolation was recorded at ages between 1-10 days (41 out of 100) flocks (41%) while lower percentage was recorded at ages between 11- 21days (8 out of 50) flocks (16%).The higher percentage of salmonella isolation from the liver (49%), while the lowest rate from yolk sac (4%). |