الفهرس 
ContentsOnly 14 pages are availabe for public view
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Abstract
Isolation and characterization Three Hortaea werneckii isolates were isolated from off shore marshes on Damietta road and were molecularly identified (by ITS1 and ITS2 sequencing), biochemically characterized (by testing different enzyme tests) and morphologically characterized (by macroscopic and microscopic examination). The result is that two strains EGYNDA08 and EGYNDA90 were alike and similar to previously recorded strain isolated from Spain Hw6, they were evolutionary relevant while EGYNDA16 is genetically distinctive and unique in its characters. Pleomorphism and polymorphism pattern The growth pattern of Hortaea werneckii strains changed by age and cell position on solid media. EGYNDA08, EGYNDA16 and EGYNDA90 were a successful model in studying pleomorphism and phenotypic response to environmental stimulants. Their molecular polymorphism was assured by applying RAPD-PCR using primers for DNA finger print and protein pattern using SDS-PAGE. The polymorphism between EGYNDA08 and EGYNDA90 was in one side and EGYNDA16 was in the other side which supported by the DNA finger printing and protein profile. Molecular and Structural Adaptation The optimum growth of EGYNDA08 and EGYNDA90 in NaCl concentration was near 0%. EGYNDA16 optimum growth was near 5% NaCl in liquid media. The fine structure of the cell under different salinities was determined by TEM examination and protein profiling using SDS-PAGE. Ultrastructural examination indicated the presence of unique structure which was present in absence of NaCl in media. Protein profile on SDS-PAGE showed differences in patterns between the isolates under different salinities.