الفهرس 
List of abbreviations
Embryology of the testisOnly 14 pages are availabe for public view
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Abstract
The present work was performed to study the microscopic, flow cytometric and morphometric alterations in the testicular tissue as well as the changes in semen of adult male albino rats after administration of caffeine for successive two months and the possible protective effect of curcumin on these changes. Fifty adult male albino rats weighing 180-200 grams were used in this study and equally divided into five main groups: group І (control group) group ІI (curcumin adminstered group) group ІІІ (caffeine administered group) group ІV (caffeine and curcumin co- administered group) group V (caffeine followed by curcumin administered group) At the end of the experiment, the surviving rats were weighed, anaesthetized intraperitoneally with sodium pentobarbital (40 mg/kg). Both testes were dissected out from each animal and were weighed and the right epididymis was obtained from each animal for semen analysis. The right testis of each animal was fixed in bouin’s fixative and 5μm thickness paraffin sections were prepared and stained with hematoxylin and eosin stain, Mallory’s trichrome stain and PAS. In addition, some paraffin sections were treated immunohistochemicaly for detection of p53.The left testis of each animal was frozen and prepared for flow cytometric ploidy test (DNA content in testis).Body weight, testicular weight, the percentage of the +ve immune-stained area for p53 and the data of both semen analysis and flow cytometric analysis were determined in both control and experimental rats and were measured statistically. from the current study, it could be concluded that that: The abuse of caffeine induced testicular damage. The degenerative effect of caffeine on testis of adult rats could be ameliorated by use of antioxidants such as curcumin. Therefore, it is recommended that: Caffeine consumption must be regulated. It is preferable to make a mixture of caffeine and curcumin which is available as dietary supplement to counteract caffeine oxidative damage.