الفهرس 
AcknowledgementOnly 14 pages are availabe for public view
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Abstract
The presented dissertation is concerned with the voltammetric, spectrodensitometric and FTIR spectroscopic analysis of certain proton pump inhibitors (PPIs) namely: omeprazole (OMZ), esomeprazole (EZM), lansoprazole (LAN), pantoprazole sodium (PAN sodium) and rabeprazole sodium (RAB sodium). It comprises six main parts Part I: Includes the general introduction about PPIs and a literature review includes the official and reported methods for their determination. Part II: This part includes a simple, rapid, accurate and sensitive SDS based voltammetric methods using pencil graphite electrode for determination of five PPIs namely: OMZ, EZM, LAN, PAN sodium and RAB sodium using sodium dodecyl sulphate as anionic surfactant. Under optimized conditions, linearity ranged from 0.009-60.0 ×10-5 M and 0.009-60.0×10-7 M for CV and SWAdSV, respectively. The detection limits for all the studied drugs ranged from 0.003-0.129×10-5 M and 0.003-0.018×10-7 M for CV and SWAdSV, respectively. The methods were successfully applied to the analysis of the studied drugs in pure form, in pharmaceutical dosage forms, biological fluids and during pharmacokinetic studies. Part III: This part includes a simple, rapid, accurate and highly sensitive surfactant complexation based voltammetric methods using pencil graphite electrode determination of five PPIs namely: OMZ, EZM, LAN, PAN sodium and RAB sodium. Under optimized conditions, linearity ranged from 0.18-10.0 ×10-7 M and 0.05-11.0×10-9 M for CV and SWAdSV, respectively. The detection limits for all the studied drugs ranged from 0.06-0.08×10-7 M and 0.015-0.070×10-9 M for CV and SWAdSV, respectively. The methods were successfully applied to the analysis of the studied drugs in pure form, in pharmaceutical dosage forms, biological fluids and during pharmacokinetic studies. Part IV: This part includes modification of pencil graphite electrode with two dyes for determination of PPIs either single or in combinations with DOM. Section A: Concerns with development of a novel, simple, accurate and sensitive voltammetric methods for determination of certain PPIs after modification of pencil graphite electrode with poly (BCG). Linearity ranges were found to be 3.3-360.0 ×10-6 M and 8.1-380.0×10-8 M for CV and SWAdSV, respectively. Limit of detection for proposed methods were 0.59-1.90 and 2.14-3.30 ×10-8 M for CV and SWAdSV, respectively. The methods were successfully applied to the analysis of the studied drugs in pure form, in pharmaceutical dosage forms, biological fluids and during pharmacokinetic studies. Section B: Concerns with development of a novel, simple, precise and sensitive voltammetric methods for simultaneous determination of certain PPIs combined with DOM after modification of pencil graphite electrode with poly (EBT). Under optimum conditions, linearity ranged from 0.45-100.0×10-7 M and 0.14-80.0 ×10-7 M for for PPIs and DOM, respectively using SWAdSV while using CV ranged from 0.69-100.0×10-6 M and 0.14-80.0×10-6 M for PPIs and DOM, respectively. The methods were successfully applied to the analysis of binary mixtures in pure form, in synthetic mixtures and biological fluids. Part V: Concerns with development of a new, simple, rapid, precise and sensitive spectrodensitometric method for separation and determination of a binary mixture containing EZM and DOM with applying fractional factorial design to show the interaction of independent variables. Linearity ranges were found to be 20-1200 and 20-600 ng/spot for EZM and DOM, respectively. Part VI: Development a simple and rapid FTIR methods for determination of a ternary and binary mixtures containing PPIs in solid state after mixing with potassium bromide Section A: Concerns with FTIR determination of ternary mixture containing OMZ, CLM and TNZ in pure forms and Helicure® capsules. The method was validated using ICH guidelines and linearity ranges were found to be 0.01-14.0, 0.15-14.0 and 0.1-8.0 %w/w for OMZ, TNZ and CLM, respectively. The limits of detection for the cited ternary mixture were 0.004, 0.05 and 0.04 % w/w for OMZ, TNZ and CLM, respectively. Section B: Concerns with FTIR determination of binary mixtures containing PPIs (OMZ, EZM, PAN sodium, LAN and RAB sodium) and DOM in pure forms and different synthetic mixtures. Linearity was achieved in concentration range of 0.02-0.80 and 0.02-0.36 % w/w for PPIs and DOM, respectively while limit of detection was achieved in the range of 0.009-0.01and 0.006-0.01% w/w for PPIs and DOM, respectively. This dissertation includes 79 figures, 7 schemes and 70 tables.