الفهرس 
titelOnly 14 pages are availabe for public view
 |  | from | 233 |  |  |
| | | from | 233 | | |
Abstract
The investigation on medicinal plants aims for searching of new and bioactive natural products, studying the chemical diversity and biochemical activity of secondary metabolites, explaining the substances which play roles in the medical use of plants as well as finding their pharmacological targets. Ipomoea aquatica Forssk. belongs to the Ipomoea genus and the Convolvulaceae family. The Ipomoea aquatica plant contains various bioactive constituents, such as phenols, flavonoids, terpenoids and saponins as well as it is used in traditional herbal medicine for leucoderma, leprosy, fever, bronchitis and liver complaints. The present study aims to develop and find the best conditions to identify the bioactive compounds from the plant’s extract in different fractions. Thus, different techniques were used for this purpose, such as HPLC and HPTLC, UPLC-QTOF/MS and LC-SPE-NMR. Results from HPLC and TLC approaches were compared. This comparison can be used as a guide to determine the best conditions for separation and purification of the active constituents from this plant by one or both approaches. The HPLC fingerprints, developed on two coupled chromolith RP-18e columns, using a gradient mobile phase starting at MeOH/H2O/TFA (5:95:0.05) (v/v/v) which showed more peaks than other tested columns and mobile phases. The HPTLC fingerprint may show less peaks than that of HPLC but it helps