الفهرس 
Review of literatureOnly 14 pages are availabe for public view
 |  | from | 145 |  |  |
| | | from | 145 | | |
Abstract
The present study included 51 paraffin blocks of invasive ductal carcinomas which were chosen from the archive of Pathology Departement, Minia University Hospital in the period between January 2012 to February 2016. All specimens were obtained by modified radical mastectomy.
We studied the immunohistochemical expression of ALDH1 and CD44 and their associations with different clinicopathological data and the correlations between the two markers.
In the present study, positive ALDH1 expression was detected in the cytoplasm of 32 cases (62.7%), and it showed a statistically significant associations with aggressive clinical and histopathological characteristics represented by larger tumor size (p= 0.039) and higher tumor grade (p=0.017). Positive ALDH1 expression was detected in all grade III tumors and those with tumor size > 5 cm in diameter.
Also, we found a statistically significant association between ALDH1 expression and extensive lymph node involvement (p= 0.030), higher LNR (p=0.023), poor NPI (P=0.021) and advanced tumor stage (p=0.039). Most of stage III tumors, tumors with > 3 positive LNs, poor NPI were positive to ALDH1 with a percentage of 81.1%, 85% and 84.2% respectively. All tumors with high LNR showed positive ALDH1 expression.
Regarding the hormone receptor status of the tumor, we found a statistically significant association between ALDH1 expression and ER, PR negativity (p=0.036, 0.011 respectively), HER2 overexpression (p=0.024) and triple negativity (p=0.017). The percentage of ER, PR, triple negative tumors expressing ALDH1 were 78.3%, 78.6% and 91.7% respectively. Most of HER2 overexpressing tumors (80.9%) were ALDH1 positive.
There was no significant association between ALDH1 expression and other clinicopathological data as: patients’ age, presence of insitu component and lymphovascular invasion.
Regarding CD44 expression, it was detected in 35 (68.6%) cases, and it showed a statistically significant associations with higher tumor grade (p= 0.037), extensive lymph node involvement (p= 0.020), higher LNR, (P=0.016), lymphovascular invasion (P=0.047) and poor NPI (P=0.003). All grade III tumors and high risk LNR were CD44 positive. Most of tumors with >3 positive LNs, positive lymphovascular invasion and poor NPI showed positive CD44 expression with a percentage of 85%, 91.7% and 84.2% respectively.
Regarding the hormonal profile of the tumors, most of ER and PR negative tumors positively expressed CD44 and their association was statistically significant (p=0.048, p=0.004 respectively). Also there was a statistically significant association between CD44 expression and HER2 overexpression (p=0.005), triple negativity (p=0.047).
There was no significant association between CD44 expression and other clinicopathological data as: patients’ age, tumor size and tumor stage.
A statistically significant positive correlation was found between ALDH1 expression and CD44 expression (p < 0.001).