الفهرس 
Introductionيوجد فقط 14 صفحة متاحة للعرض العام
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المستخلص
Urinary tracts infections are silly problems make individual upset and can lead to serious complications, even organ damage. Commonly referred to as UTIs, the infection primarily targets the bladder and kidneys and has a nasty tendency for recurrence. Women, teens, and young children are most often afflicted but men may suffer from them as well. While UTI’s may seem like a straightforward infection for which antibiotics are the sole solution. Many of the bacteria which cause UTIs have developed resistance to antibiotics and the impact is alarming. Multi-drug resistant pathogens, or MDRs, scoff at typical antibiotics and need to be antagonized by a more aggressive, heavy-duty profile. Enterococci bacteria show a special resistance to standard antibiotics. Those who suffer from recurring UTIs face the problem of increased infection by antibiotic resistant bugs. Clearly, prevention is best measure in the battle against UTIs. With the ineffectiveness of antibiotics, research has turned to probiotics as an alternative mean to address UTIs. Lactobacillus probiotic strains have reported the promising results. In one study, patients who took this strain experienced significant improvements. These particular probiotic bacteria can also stimulate immune function, lower acidity levels in the urinary tract and deactivate the growth of UTI causing organisms.
In this study, two probiotics were examined for their ability to inhibit eight urogenital pathogens. In preliminary experiments, all pathogenic isolates were identified by the available biochemical tests. Their ability to afford different temperatures and a wide range of pH and their effect on blood agar are also tested.
The antibiotic susceptibility testing was determined against 13 different antimicrobial agents representing different classes of antibiotics by disc agar diffusion technique. The resistance pattern showed that all isolates were completely resistant to azithromycin (AZM), cefepime (FEP), cefoperazone (CFP), erythromycin (E), ofloxacin (OFX) and levofloxacin (LEV). Uropathogenic isolates were sensitive to imipenem (IPM) 52.9%, while 47% of isolates have an intermediate sensitivity to amikacin (AK), 94.1% of isolates were resistant to rifampin (RF) and gentamicin (CN), 97% were resistant to cefotaxime (CTX) and amoxicillin (AMC) and 70.5% were resistant to ceftriaxone (CRO).
Twenty five lactic acid bacteria were isolated from different sources of pickles, mixed pickles, yogurt and sausage. The morphological characteristics of the isolated LAB were determined. Then, the well agar diffusion method was used to determine the inhibitory effect of the 25 isolated lactic acid bacteria. The results were disappointing as they showed no inhibitory effect against the isolated uropathogens. So, we resort to two identified strains (E. faecium NM2 and L. plantarum LPS10) to complete our study. The diameter of the clear zone appeared is measured after incubation. This method clarified that L. plantarum LPS10 has an inhibitory effect on S aureus (18mm), E. coli (15mm),Shigella Sp.(13mm) , K. Pneumoniae (12mm),C. albicans(11mm) and P. vulgaris (8mm) , while E. faecium NM2 has an inhibitory effect only on Shigella sp.(11mm), E. coli (9mm), S. aureus (6mm) , K. Pneumoniae (3mm) and C. albicans (8mm).
The two identified probiotics (E. faecium NM2 and L. plantarum LPS10) were used to study their antagonistic effect against isolated uropathogens by another technique. The CFS of both probiotics was used
In studying their effect on the 8 isolated uropathogens in a liquid media. Results were promising as L. plantarum LPS10 completely inhibited the growth of S. aureus and E.coli after 24hs and 48hs respectively. On the other hand, both probiotics could decrease the growth of other pathogens by 2 log cycles at different intervals. The difference in colony count (CFU/ml) between the control experiments and treated samples with LPS10 and NM2 was nearly 3-4 log cycles within 96hs. This is reflecting the strong inhibitory effect of the two probiotics on the isolated uropathogens.